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Synthetic Three-Component HIV-1 V3 Glycopeptide Immunogens Induce Glycan-Dependent Antibody Responses.

Publication ,  Journal Article
Cai, H; Orwenyo, J; Giddens, JP; Yang, Q; Zhang, R; LaBranche, CC; Montefiori, DC; Wang, L-X
Published in: Cell Chem Biol
December 21, 2017

Eliciting broadly neutralizing antibody (bNAb) responses against HIV-1 is a major goal for a prophylactic HIV-1 vaccine. One approach is to design immunogens based on known broadly neutralizing epitopes. Here we report the design and synthesis of an HIV-1 glycopeptide immunogen derived from the V3 domain. We performed glycopeptide epitope mapping to determine the minimal glycopeptide sequence as the epitope of V3-glycan-specific bNAbs PGT128 and 10-1074. We further constructed a self-adjuvant three-component immunogen that consists of a 33-mer V3 glycopeptide epitope, a universal T helper epitope P30, and a lipopeptide (Pam3CSK4) that serves as a ligand of Toll-like receptor 2. Rabbit immunization revealed that the synthetic self-adjuvant glycopeptide could elicit substantial glycan-dependent antibodies that exhibited broader recognition of HIV-1 gp120s than the non-glycosylated V3 peptide. These results suggest that the self-adjuvant synthetic glycopeptides can serve as an important component to elicit glycan-specific antibodies in HIV vaccine design.

Duke Scholars

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Published In

Cell Chem Biol

DOI

EISSN

2451-9448

Publication Date

December 21, 2017

Volume

24

Issue

12

Start / End Page

1513 / 1522.e4

Location

United States

Related Subject Headings

  • Rabbits
  • Polysaccharides
  • HIV Envelope Protein gp120
  • Epitopes
  • Antibody Formation
  • Antibodies
  • Animals
 

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Cai, H., Orwenyo, J., Giddens, J. P., Yang, Q., Zhang, R., LaBranche, C. C., … Wang, L.-X. (2017). Synthetic Three-Component HIV-1 V3 Glycopeptide Immunogens Induce Glycan-Dependent Antibody Responses. Cell Chem Biol, 24(12), 1513-1522.e4. https://doi.org/10.1016/j.chembiol.2017.09.005
Cai, Hui, Jared Orwenyo, John P. Giddens, Qiang Yang, Roushu Zhang, Celia C. LaBranche, David C. Montefiori, and Lai-Xi Wang. “Synthetic Three-Component HIV-1 V3 Glycopeptide Immunogens Induce Glycan-Dependent Antibody Responses.Cell Chem Biol 24, no. 12 (December 21, 2017): 1513-1522.e4. https://doi.org/10.1016/j.chembiol.2017.09.005.
Cai H, Orwenyo J, Giddens JP, Yang Q, Zhang R, LaBranche CC, et al. Synthetic Three-Component HIV-1 V3 Glycopeptide Immunogens Induce Glycan-Dependent Antibody Responses. Cell Chem Biol. 2017 Dec 21;24(12):1513-1522.e4.
Cai, Hui, et al. “Synthetic Three-Component HIV-1 V3 Glycopeptide Immunogens Induce Glycan-Dependent Antibody Responses.Cell Chem Biol, vol. 24, no. 12, Dec. 2017, pp. 1513-1522.e4. Pubmed, doi:10.1016/j.chembiol.2017.09.005.
Cai H, Orwenyo J, Giddens JP, Yang Q, Zhang R, LaBranche CC, Montefiori DC, Wang L-X. Synthetic Three-Component HIV-1 V3 Glycopeptide Immunogens Induce Glycan-Dependent Antibody Responses. Cell Chem Biol. 2017 Dec 21;24(12):1513-1522.e4.

Published In

Cell Chem Biol

DOI

EISSN

2451-9448

Publication Date

December 21, 2017

Volume

24

Issue

12

Start / End Page

1513 / 1522.e4

Location

United States

Related Subject Headings

  • Rabbits
  • Polysaccharides
  • HIV Envelope Protein gp120
  • Epitopes
  • Antibody Formation
  • Antibodies
  • Animals