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Nonionic Ethoxylated Surfactants Induce Adipogenesis in 3T3-L1 Cells.

Publication ,  Journal Article
Kassotis, CD; Kollitz, EM; Ferguson, PL; Stapleton, HM
Published in: Toxicological sciences : an official journal of the Society of Toxicology
March 2018

Recent studies have demonstrated that a number of environmental contaminants can act as metabolic disruptors and modulate metabolic function both in vitro and in vivo. 3T3-L1 mouse preadipocytes are commonly utilized to assess perturbations to adipogenesis, providing insight into environmental contaminants that may impact in vivo metabolic health. This study sought to assess whether various alkylphenol ethoxylates and alcohol ethoxylates (APEOs and AEOs, respectively), ubiquitous contaminants used in common household products, could disrupt metabolic health. 3T3-L1 cells were exposed to increasing concentrations of individual ethoxylated surfactants and base hydrophobes, and assessed for triglyceride accumulation (relative to a rosiglitazone-induced maximum response) and preadipocyte proliferation (relative to a differentiated vehicle control). We report herein that nonionic APEOs and AEOs promoted triglyceride accumulation and/or preadipocyte proliferation in 3T3-L1 cells at concentrations from 0.1 to 10 μM. Activity appeared to be an effect of the polyethoxylate chain length, as the alkylphenol/alcohol hydrophobes exhibited minimal or no adipogenic activity. In addition, nonylphenol ethoxylates (NPEO) of various ethoxylate chain lengths exhibited biphasic adipogenic activity, with increasing triglyceride accumulation and preadipocyte proliferation from NPEO (0, average ethoxylate number) through NPEO (4), and then decreasing activities from NPEO (4) through NPEO (20). Our results suggest potential metabolic impacts of these compounds at environmentally relevant concentrations, demonstrating a need to further assess molecular mechanisms and better characterize environmental concentrations of the specific AEOs and APEOs that are inducing the greatest degree of adipogenic activity herein.

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Published In

Toxicological sciences : an official journal of the Society of Toxicology

DOI

EISSN

1096-0929

ISSN

1096-6080

Publication Date

March 2018

Volume

162

Issue

1

Start / End Page

124 / 136

Related Subject Headings

  • Triglycerides
  • Toxicology
  • Surface-Active Agents
  • Phenols
  • Mice
  • Ethers
  • Environmental Pollutants
  • Cell Differentiation
  • Animals
  • Alcohols
 

Citation

APA
Chicago
ICMJE
MLA
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Kassotis, C. D., Kollitz, E. M., Ferguson, P. L., & Stapleton, H. M. (2018). Nonionic Ethoxylated Surfactants Induce Adipogenesis in 3T3-L1 Cells. Toxicological Sciences : An Official Journal of the Society of Toxicology, 162(1), 124–136. https://doi.org/10.1093/toxsci/kfx234
Kassotis, Christopher D., Erin M. Kollitz, Patrick Lee Ferguson, and Heather M. Stapleton. “Nonionic Ethoxylated Surfactants Induce Adipogenesis in 3T3-L1 Cells.Toxicological Sciences : An Official Journal of the Society of Toxicology 162, no. 1 (March 2018): 124–36. https://doi.org/10.1093/toxsci/kfx234.
Kassotis CD, Kollitz EM, Ferguson PL, Stapleton HM. Nonionic Ethoxylated Surfactants Induce Adipogenesis in 3T3-L1 Cells. Toxicological sciences : an official journal of the Society of Toxicology. 2018 Mar;162(1):124–36.
Kassotis, Christopher D., et al. “Nonionic Ethoxylated Surfactants Induce Adipogenesis in 3T3-L1 Cells.Toxicological Sciences : An Official Journal of the Society of Toxicology, vol. 162, no. 1, Mar. 2018, pp. 124–36. Epmc, doi:10.1093/toxsci/kfx234.
Kassotis CD, Kollitz EM, Ferguson PL, Stapleton HM. Nonionic Ethoxylated Surfactants Induce Adipogenesis in 3T3-L1 Cells. Toxicological sciences : an official journal of the Society of Toxicology. 2018 Mar;162(1):124–136.
Journal cover image

Published In

Toxicological sciences : an official journal of the Society of Toxicology

DOI

EISSN

1096-0929

ISSN

1096-6080

Publication Date

March 2018

Volume

162

Issue

1

Start / End Page

124 / 136

Related Subject Headings

  • Triglycerides
  • Toxicology
  • Surface-Active Agents
  • Phenols
  • Mice
  • Ethers
  • Environmental Pollutants
  • Cell Differentiation
  • Animals
  • Alcohols