Endothelial nitric oxide synthase stimulates aneurysm growth in aged mice.

Published

Journal Article

Age-associated changes in endothelial nitric oxide synthase (eNOS) expression have not been definitively linked to the pathophysiology of aortic aneurysms. We examined the role of eNOS in human patients and an age-appropriate mouse model.eNOS transcripts and immunodetectable protein were assessed by quantitative PCR and immunohistochemistry in human ascending thoracic aneurysms (n = 29) and referent aortae (n = 31). Carotid aneurysms were induced with CaCl2 in young adult (3 months) and aged (18 months) C57BL/6 and eNOS-knockout (eNOS-KO) mice.eNOS transcripts and protein were reduced in human aneurysms compared with controls, although aortic eNOS expression also decreased with patient age. Aged wild-type mice had significantly larger aneurysm diameter than young adult mice. Aged wild-type mice had reduced eNOS transcripts and protein compared with young adult mice. Aged eNOS-KO mice had smaller aneurysms compared with aged wild-type mice but similar size aneurysms compared with young eNOS-KO and young wild-type mice.eNOS expression is reduced in both aged human and aged mouse endothelium and eNOS expression is linked to aneurysm expansion in aged but not young adult mice. These findings support the relevance of age-associated changes in eNOS expression in clinical aneurysmal disease.

Full Text

Cited Authors

  • Pimiento, JM; Maloney, SP; Tang, PCY; Muto, A; Westvik, TS; Fitzgerald, TN; Fancher, TT; Tellides, G; Dardik, A

Published Date

  • January 10, 2008

Published In

Volume / Issue

  • 45 / 3

Start / End Page

  • 251 - 258

PubMed ID

  • 18182824

Pubmed Central ID

  • 18182824

Electronic International Standard Serial Number (EISSN)

  • 1423-0135

International Standard Serial Number (ISSN)

  • 1018-1172

Digital Object Identifier (DOI)

  • 10.1159/000112940

Language

  • eng