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Expression of Mitochondrial-Encoded Genes in Blood Differentiate Acute Renal Allograft Rejection.

Publication ,  Journal Article
Roedder, S; Sigdel, T; Hsieh, S-C; Cheeseman, J; Metes, D; Macedo, C; Reed, EF; Gritsch, HA; Zeevi, A; Shapiro, R; Kirk, AD; Sarwal, MM
Published in: Front Med (Lausanne)
2017

Despite potent immunosuppression, clinical and biopsy confirmed acute renal allograft rejection (AR) still occurs in 10-15% of recipients, ~30% of patients demonstrate subclinical rejection on biopsy, and ~50% of them can show molecular inflammation, all which increase the risk of chronic dysfunction and worsened allograft outcomes. Mitochondria represent intracellular endogenous triggers of inflammation, which can regulate immune cell differentiation, and expansion and cause antigen-independent graft injury, potentially enhancing the development of acute rejection. In the present study, we investigated the role of mitochondrial DNA encoded gene expression in biopsy matched peripheral blood (PB) samples from kidney transplant recipients. Quantitative PCR was performed in 155 PB samples from 115 unique pediatric (<21 years) and adult (>21 years) renal allograft recipients at the point of AR (n = 61) and absence of rejection (n = 94) for the expression of 11 mitochondrial DNA encoded genes. We observed increased expression of all genes in adult recipients compared to pediatric recipients; separate analyses in both cohorts demonstrated increased expression during rejection, which also differentiated borderline rejection and showed an increasing pattern in serially collected samples (0-3 months prior to and post rejection). Our results provide new insights on the role of mitochondria during rejection and potentially indicate mitochondria as targets for novel immunosuppression.

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Published In

Front Med (Lausanne)

DOI

ISSN

2296-858X

Publication Date

2017

Volume

4

Start / End Page

185

Location

Switzerland

Related Subject Headings

  • 42 Health sciences
  • 32 Biomedical and clinical sciences
 

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Roedder, S., Sigdel, T., Hsieh, S.-C., Cheeseman, J., Metes, D., Macedo, C., … Sarwal, M. M. (2017). Expression of Mitochondrial-Encoded Genes in Blood Differentiate Acute Renal Allograft Rejection. Front Med (Lausanne), 4, 185. https://doi.org/10.3389/fmed.2017.00185
Roedder, Silke, Tara Sigdel, Szu-Chuan Hsieh, Jennifer Cheeseman, Diana Metes, Camila Macedo, Elaine F. Reed, et al. “Expression of Mitochondrial-Encoded Genes in Blood Differentiate Acute Renal Allograft Rejection.Front Med (Lausanne) 4 (2017): 185. https://doi.org/10.3389/fmed.2017.00185.
Roedder S, Sigdel T, Hsieh S-C, Cheeseman J, Metes D, Macedo C, et al. Expression of Mitochondrial-Encoded Genes in Blood Differentiate Acute Renal Allograft Rejection. Front Med (Lausanne). 2017;4:185.
Roedder, Silke, et al. “Expression of Mitochondrial-Encoded Genes in Blood Differentiate Acute Renal Allograft Rejection.Front Med (Lausanne), vol. 4, 2017, p. 185. Pubmed, doi:10.3389/fmed.2017.00185.
Roedder S, Sigdel T, Hsieh S-C, Cheeseman J, Metes D, Macedo C, Reed EF, Gritsch HA, Zeevi A, Shapiro R, Kirk AD, Sarwal MM. Expression of Mitochondrial-Encoded Genes in Blood Differentiate Acute Renal Allograft Rejection. Front Med (Lausanne). 2017;4:185.

Published In

Front Med (Lausanne)

DOI

ISSN

2296-858X

Publication Date

2017

Volume

4

Start / End Page

185

Location

Switzerland

Related Subject Headings

  • 42 Health sciences
  • 32 Biomedical and clinical sciences