Packed capillary liquid chromatography/electrospray ionization/mass spectrometry in the pharmaceutical sciences: Characterization of protein mixtures
Publication
, Journal Article
Moseley, MA; Unger, SE
Published in: Journal of Microcolumn Separations
January 1, 1992
The coupling of packed capillary liquid chromatography with electrospray ionization mass spectrometry has yielded an instrument capable of generating molecular weight information from very complex natural protein mixtures. The use of the packed capillary liquid chromatography columns offers two principle advantages over conventional liquid chromatography columns: high separation efficiencies and very low mobile phase flow rates (100 nL min−1). The low mobile phase flow rates permit the use of a relatively high ionic strength buffer (0.1% trifluoroacetic acid), While delivering a very low mass flux of the buffer to the mass spectrometer. Copyright © 1992 MicroSeparations Incorporated
Duke Scholars
Published In
Journal of Microcolumn Separations
DOI
EISSN
1520-667X
ISSN
1040-7685
Publication Date
January 1, 1992
Volume
4
Issue
5
Start / End Page
393 / 398
Related Subject Headings
- Analytical Chemistry
- 0601 Biochemistry and Cell Biology
- 0399 Other Chemical Sciences
- 0301 Analytical Chemistry
Citation
APA
Chicago
ICMJE
MLA
NLM
Moseley, M. A., & Unger, S. E. (1992). Packed capillary liquid chromatography/electrospray ionization/mass spectrometry in the pharmaceutical sciences: Characterization of protein mixtures. Journal of Microcolumn Separations, 4(5), 393–398. https://doi.org/10.1002/mcs.1220040505
Moseley, M. A., and S. E. Unger. “Packed capillary liquid chromatography/electrospray ionization/mass spectrometry in the pharmaceutical sciences: Characterization of protein mixtures.” Journal of Microcolumn Separations 4, no. 5 (January 1, 1992): 393–98. https://doi.org/10.1002/mcs.1220040505.
Moseley MA, Unger SE. Packed capillary liquid chromatography/electrospray ionization/mass spectrometry in the pharmaceutical sciences: Characterization of protein mixtures. Journal of Microcolumn Separations. 1992 Jan 1;4(5):393–8.
Moseley, M. A., and S. E. Unger. “Packed capillary liquid chromatography/electrospray ionization/mass spectrometry in the pharmaceutical sciences: Characterization of protein mixtures.” Journal of Microcolumn Separations, vol. 4, no. 5, Jan. 1992, pp. 393–98. Scopus, doi:10.1002/mcs.1220040505.
Moseley MA, Unger SE. Packed capillary liquid chromatography/electrospray ionization/mass spectrometry in the pharmaceutical sciences: Characterization of protein mixtures. Journal of Microcolumn Separations. 1992 Jan 1;4(5):393–398.
Published In
Journal of Microcolumn Separations
DOI
EISSN
1520-667X
ISSN
1040-7685
Publication Date
January 1, 1992
Volume
4
Issue
5
Start / End Page
393 / 398
Related Subject Headings
- Analytical Chemistry
- 0601 Biochemistry and Cell Biology
- 0399 Other Chemical Sciences
- 0301 Analytical Chemistry