Different roles of radical scavengers--ascorbate and urate in the cerebrospinal fluid of amyotrophic lateral sclerosis patients.


Journal Article

Ferrous iron, released from iron deposits in the motor cortex and other brain regions of amyotrophic lateral sclerosis (ALS) patients, participates in the Fenton reaction in cerebrospinal fluid (CSF) alongside H(2)O(2), which is continuously released by neuronal cells. In vivo, the production of notoriously reactive hydroxyl radicals via this reaction could lead to the progression of the disease. Herein, we have examined the effect of ascorbate and uric acid on the production of hydroxyl radicals in CSF from both sporadic ALS patients and control subjects. Electron paramagnetic resonance spectroscopy identified ascorbyl radicals in CSF from ALS patients whereas it was undetectable in control CSF. The addition of H(2)O(2) to the CSF from ALS patients provoked further formation of ascorbyl radicals and the formation of hydroxyl radicals ex vivo. The hydroxyl addition of uric acid to CSF from ALS patients diminished the production of hydroxyl radicals. In conclusion, there are clear differences between the roles of the two examined radical scavengers in the CSF of ALS patients indicating that the use of ascorbate could have unfavourable effects in ALS patients.

Full Text

Duke Authors

Cited Authors

  • Spasojević, I; Stević, Z; Nikolić-Kokić, A; Jones, DR; Blagojević, D; Spasić, MB

Published Date

  • 2010

Published In

Volume / Issue

  • 15 / 2

Start / End Page

  • 81 - 86

PubMed ID

  • 20500989

Pubmed Central ID

  • 20500989

Electronic International Standard Serial Number (EISSN)

  • 1743-2928

Digital Object Identifier (DOI)

  • 10.1179/174329210X12650506623320


  • eng

Conference Location

  • England