Detection of hydrogen atom adduct of spin-trap DEPMPO. The relevance for studies of biological systems.

Published

Conference Paper

We proposed EPR spectroscopy using spin-trap DEPMPO as a novel method for the detection of a hydrogen atom (*H) produced by chemical and biological systems. In complex EPR spectra of DEPMPO adducts in biological systems, spectral lines of unknown origin have been observed. We have assumed (Bacić, G.; Mojović, M. Ann. N. Y. Acad. Sci. 2005, 1048, 230-243) that those lines represent the spectrum of a hydrogen atom (*H) adduct i.e., DEPMPO/H. An electrochemical system known to produce only *H radicals was used here in order to obtain a separate spectrum of the DEPMPO/H adduct. An acquired spectrum as well as a computer spectral simulation of the DEPMPO/H adduct showed considerable resemblance with additional lines in the EPR spectra of DEPMPO adducts in biological systems-plant plasma membranes and cell walls. This shows that such a radical is produced by plants as well as that DEPMPO is suitable for detection in both electrochemical and biological systems.

Full Text

Duke Authors

Cited Authors

  • Mojović, M; Spasojević, I; Bacić, G

Published Date

  • November 2005

Published In

Volume / Issue

  • 45 / 6

Start / End Page

  • 1716 - 1718

PubMed ID

  • 16309278

Pubmed Central ID

  • 16309278

International Standard Serial Number (ISSN)

  • 1549-9596

Digital Object Identifier (DOI)

  • 10.1021/ci050173d

Conference Location

  • United States