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Increased surface expression of HIV-1 envelope is associated with improved antibody response in vaccinia prime/protein boost immunization.

Publication ,  Journal Article
Hogan, MJ; Conde-Motter, A; Jordan, APO; Yang, L; Cleveland, B; Guo, W; Romano, J; Ni, H; Pardi, N; LaBranche, CC; Montefiori, DC; Hu, S-L ...
Published in: Virology
January 15, 2018

HIV-1 envelope (Env)-based vaccines have so far largely failed to induce antibodies that prevent HIV-1 infection. One factor proposed to limit the immunogenicity of cell-associated Env is its low level of expression on the cell surface, restricting accessibility to antibodies. Using a vaccinia prime/protein boost protocol in mice, we explored the immunologic effects of mutations in the Env cytoplasmic tail (CT) that increased surface expression, including partial truncation and ablation of a tyrosine-dependent endocytosis motif. After vaccinia primes, CT-modified Envs induced up to 7-fold higher gp120-specific IgG, and after gp120 protein boosts, they elicited up to 16-fold greater Tier-1 HIV-1 neutralizing antibody titers, although results were variable between isolates. These data indicate that the immunogenicity of HIV-1 Env in a prime/boost vaccine can be enhanced in a strain-dependent manner by CT mutations that increase Env surface expression, thus highlighting the importance of the prime in this vaccine format.

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Published In

Virology

DOI

EISSN

1096-0341

Publication Date

January 15, 2018

Volume

514

Start / End Page

106 / 117

Location

United States

Related Subject Headings

  • Virology
  • Vaccinia virus
  • Mice, Inbred C57BL
  • Mice
  • Immunoglobulin G
  • Immunization, Secondary
  • Humans
  • HIV-1
  • HIV Infections
  • HIV Envelope Protein gp120
 

Citation

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Hogan, M. J., Conde-Motter, A., Jordan, A. P. O., Yang, L., Cleveland, B., Guo, W., … Weissman, D. (2018). Increased surface expression of HIV-1 envelope is associated with improved antibody response in vaccinia prime/protein boost immunization. Virology, 514, 106–117. https://doi.org/10.1016/j.virol.2017.10.013
Hogan, Michael J., Angela Conde-Motter, Andrea P. O. Jordan, Lifei Yang, Brad Cleveland, Wenjin Guo, Josephine Romano, et al. “Increased surface expression of HIV-1 envelope is associated with improved antibody response in vaccinia prime/protein boost immunization.Virology 514 (January 15, 2018): 106–17. https://doi.org/10.1016/j.virol.2017.10.013.
Hogan MJ, Conde-Motter A, Jordan APO, Yang L, Cleveland B, Guo W, et al. Increased surface expression of HIV-1 envelope is associated with improved antibody response in vaccinia prime/protein boost immunization. Virology. 2018 Jan 15;514:106–17.
Hogan, Michael J., et al. “Increased surface expression of HIV-1 envelope is associated with improved antibody response in vaccinia prime/protein boost immunization.Virology, vol. 514, Jan. 2018, pp. 106–17. Pubmed, doi:10.1016/j.virol.2017.10.013.
Hogan MJ, Conde-Motter A, Jordan APO, Yang L, Cleveland B, Guo W, Romano J, Ni H, Pardi N, LaBranche CC, Montefiori DC, Hu S-L, Hoxie JA, Weissman D. Increased surface expression of HIV-1 envelope is associated with improved antibody response in vaccinia prime/protein boost immunization. Virology. 2018 Jan 15;514:106–117.
Journal cover image

Published In

Virology

DOI

EISSN

1096-0341

Publication Date

January 15, 2018

Volume

514

Start / End Page

106 / 117

Location

United States

Related Subject Headings

  • Virology
  • Vaccinia virus
  • Mice, Inbred C57BL
  • Mice
  • Immunoglobulin G
  • Immunization, Secondary
  • Humans
  • HIV-1
  • HIV Infections
  • HIV Envelope Protein gp120