Nigrotectal Stimulation Stops Interval Timing in Mice.

Published

Journal Article

Considerable evidence implicates the basal ganglia in interval timing, yet the underlying mechanisms remain poorly understood. Using a novel behavioral task, we demonstrate that head-fixed mice can be trained to show the key features of timing behavior within a few sessions. Single-trial analysis of licking behavior reveals stepping dynamics with variable onset times, which is responsible for the canonical Gaussian distribution of timing behavior. Moreover, the duration of licking bouts decreased as mice became sated, showing a strong motivational modulation of licking bout initiation and termination. Using optogenetics, we examined the role of the basal ganglia output in interval timing. We stimulated a pathway important for licking behavior, the GABAergic output projections from the substantia nigra pars reticulata to the deep layers of the superior colliculus. We found that stimulation of this pathway not only cancelled licking but also delayed the initiation of anticipatory licking for the next interval in a frequency-dependent manner. By combining quantitative behavioral analysis with optogenetics in the head-fixed setup, we established a new approach for studying the neural basis of interval timing.

Full Text

Duke Authors

Cited Authors

  • Toda, K; Lusk, NA; Watson, GDR; Kim, N; Lu, D; Li, HE; Meck, WH; Yin, HH

Published Date

  • December 2017

Published In

Volume / Issue

  • 27 / 24

Start / End Page

  • 3763 - 3770.e3

PubMed ID

  • 29199075

Pubmed Central ID

  • 29199075

Electronic International Standard Serial Number (EISSN)

  • 1879-0445

International Standard Serial Number (ISSN)

  • 0960-9822

Digital Object Identifier (DOI)

  • 10.1016/j.cub.2017.11.003

Language

  • eng