Bleb Expansion in Migrating Cells Depends on Supply of Membrane from Cell Surface Invaginations.

Journal Article

Cell migration is essential for morphogenesis, organ formation, and homeostasis, with relevance for clinical conditions. The migration of primordial germ cells (PGCs) is a useful model for studying this process in the context of the developing embryo. Zebrafish PGC migration depends on the formation of cellular protrusions in form of blebs, a type of protrusion found in various cell types. Here we report on the mechanisms allowing the inflation of the membrane during bleb formation. We show that the rapid expansion of the protrusion depends on membrane invaginations that are localized preferentially at the cell front. The formation of these invaginations requires the function of Cdc42, and their unfolding allows bleb inflation and dynamic cell-shape changes performed by migrating cells. Inhibiting the formation and release of the invaginations strongly interfered with bleb formation, cell motility, and the ability of the cells to reach their target.

Full Text

Duke Authors

Cited Authors

  • Goudarzi, M; Tarbashevich, K; Mildner, K; Begemann, I; Garcia, J; Paksa, A; Reichman-Fried, M; Mahabaleshwar, H; Blaser, H; Hartwig, J; Zeuschner, D; Galic, M; Bagnat, M; Betz, T; Raz, E

Published Date

  • December 2017

Published In

Volume / Issue

  • 43 / 5

Start / End Page

  • 577 - 587.e5

PubMed ID

  • 29173819

Electronic International Standard Serial Number (EISSN)

  • 1878-1551

International Standard Serial Number (ISSN)

  • 1534-5807

Digital Object Identifier (DOI)

  • 10.1016/j.devcel.2017.10.030

Language

  • eng