Zebrafish Transcription Factor ORFeome for Gene Discovery and Regulatory Network Elucidation.


Journal Article

The completion of the zebrafish genome sequence and advances in miniaturization and multiplexing were essential to the creation of techniques such as RNA-seq, ChIP-seq, and high-throughput behavioral and chemical screens. Multiplexing was also instrumental in the recent enhancement of the classic yeast one-hybrid interaction techniques to provide unprecedented discovery capabilities for protein-DNA interactions. Unfortunately its use for zebrafish research is currently hampered by the lack of an open reading frame (ORF) clone collection. As a first step toward a complete collection, we describe a small library of transcriptional regulatory proteins comprising 142 ORFs and its potential applications.

Full Text

Duke Authors

Cited Authors

  • King, J; Foster, J; Davison, JM; Rawls, JF; Breton, G

Published Date

  • April 2018

Published In

Volume / Issue

  • 15 / 2

Start / End Page

  • 202 - 205

PubMed ID

  • 29173090

Pubmed Central ID

  • 29173090

Electronic International Standard Serial Number (EISSN)

  • 1557-8542

Digital Object Identifier (DOI)

  • 10.1089/zeb.2017.1486


  • eng

Conference Location

  • United States