1 H-nmr metabolomic study of whole blood from hatchling loggerhead sea turtles (Caretta caretta) exposed to crude oil and/or corexit


Journal Article

© 2017 The Authors. We used proton nuclear magnetic resonance spectroscopy (1H-NMR) to evaluate metabolic impacts of environmentally relevant crude oil and Corexit exposures on the physiology of hatchling loggerhead sea turtles (Caretta caretta). Sample extraction and data acquisition methods for very small volume whole blood samples and sources of variation between individual hatchlings were assessed. Sixteen unclotted, whole blood samples were obtained from 7-day-old hatchlings after a 4-day cutaneous exposure to either control seawater, crude oil, Corexit 9500A or a combination of crude oil and Corexit 9500A. After extraction, one- and two-dimensional 1H-NMR spectra of the samples were obtained, and 17 metabolites were identified and confirmed in the whole blood spectra. Variation among samples due to the concentrations of metabolites 3-hydroxybutyrate, lactate, trimethylamine oxide and propylene glycol did not statistically correlate with treatment group. However, the characterization of the hatchling loggerhead whole blood metabolome provides a foundation for future metabolomic research with sea turtles and a basis for the study of tissues from exposed hatchling sea turtles.

Full Text

Duke Authors

Cited Authors

  • Bembenek Bailey, SA; Niemuth, JN; McClellan-Green, PD; Godfrey, MH; Harms, CA; Stoskopf, MK

Published Date

  • November 22, 2017

Published In

Volume / Issue

  • 4 / 11

Electronic International Standard Serial Number (EISSN)

  • 2054-5703

Digital Object Identifier (DOI)

  • 10.1098/rsos.171433

Citation Source

  • Scopus