Localization of structural proteins in African swine fever virus particles by immunoelectron microscopy.

Published

Journal Article

Seven African swine fever virus structural proteins were localized in the virion by immunoelectron microscopy. African swine fever virus-infected cells were incubated, before or after embedding and thin sectioning, with monoclonal antibodies specific for different structural proteins, and after labeling with protein A-gold complexes, the samples were examined in the electron microscope. Proteins p14 and p24 were found in the external region of the virion, proteins p12, p72, p17, and p37 were found in the intermediate layers, and protein p150 was found in the nucleoid and in one vertex. A monoclonal antibody that recognized protein p150 as well as p220, a virus-induced, nonstructural protein, could also bind to a component present in the nucleus of both uninfected and virus-infected cells.

Full Text

Duke Authors

Cited Authors

  • Carrascosa, JL; González, P; Carrascosa, AL; Garciá-Barreno, B; Enjuanes, L; Viñuela, E

Published Date

  • May 1, 1986

Published In

Volume / Issue

  • 58 / 2

Start / End Page

  • 377 - 384

PubMed ID

  • 3517383

Pubmed Central ID

  • 3517383

Electronic International Standard Serial Number (EISSN)

  • 1098-5514

International Standard Serial Number (ISSN)

  • 0022-538X

Language

  • eng