Mutation analysis of 24 known cancer genes in the NCI-60 cell line set.

Published

Journal Article

The panel of 60 human cancer cell lines (the NCI-60) assembled by the National Cancer Institute for anticancer drug discovery is a widely used resource. The NCI-60 has been characterized pharmacologically and at the molecular level more extensively than any other set of cell lines. However, no systematic mutation analysis of genes causally implicated in oncogenesis has been reported. This study reports the sequence analysis of 24 known cancer genes in the NCI-60 and an assessment of 4 of the 24 genes for homozygous deletions. One hundred thirty-seven oncogenic mutations were identified in 14 (APC, BRAF, CDKN2, CTNNB1, HRAS, KRAS, NRAS, SMAD4, PIK3CA, PTEN, RB1, STK11, TP53, and VHL) of the 24 genes. All lines have at least one mutation among the cancer genes examined, with most lines (73%) having more than one. Identification of those cancer genes mutated in the NCI-60, in combination with pharmacologic and molecular profiles of the cells, will allow for more informed interpretation of anticancer agent screening and will enhance the use of the NCI-60 cell lines for molecularly targeted screens.

Full Text

Duke Authors

Cited Authors

  • Ikediobi, ON; Davies, H; Bignell, G; Edkins, S; Stevens, C; O'Meara, S; Santarius, T; Avis, T; Barthorpe, S; Brackenbury, L; Buck, G; Butler, A; Clements, J; Cole, J; Dicks, E; Forbes, S; Gray, K; Halliday, K; Harrison, R; Hills, K; Hinton, J; Hunter, C; Jenkinson, A; Jones, D; Kosmidou, V; Lugg, R; Menzies, A; Mironenko, T; Parker, A; Perry, J; Raine, K; Richardson, D; Shepherd, R; Small, A; Smith, R; Solomon, H; Stephens, P; Teague, J; Tofts, C; Varian, J; Webb, T; West, S; Widaa, S; Yates, A; Reinhold, W; Weinstein, JN; Stratton, MR; Futreal, PA; Wooster, R

Published Date

  • November 6, 2006

Published In

Volume / Issue

  • 5 / 11

Start / End Page

  • 2606 - 2612

PubMed ID

  • 17088437

Pubmed Central ID

  • 17088437

Electronic International Standard Serial Number (EISSN)

  • 1538-8514

International Standard Serial Number (ISSN)

  • 1535-7163

Digital Object Identifier (DOI)

  • 10.1158/1535-7163.MCT-06-0433

Language

  • eng