Differential orientation of 10T1/2 mesenchymal cells on non-uniform stretch environments.

Published

Journal Article

Non-uniform stress and strain fields are prevalent in many tissues in vivo, and often exacerbated by disease or injury. These mechanical gradients potentially play a role in contributing to pathological conditions, presenting a need for experimental tools to allow investigation of cell behavior within non-uniformly stimulated environments. Herein, we employ two in vitro cell-stretching devices (one previously published; one newly presented) capable of subjecting cells to cyclic, non-uniform stretches upon the surface of either a circular elastomeric membrane or a cylindrical PDMS tube. After 24 hours of cyclic stretch, 10T1/2 cells on both devices showed marked changes in long-axis orientation, with tendencies to align parallel to the direction of minimal deformation. The degree of this response varied depending on location within the stretch gradients. These results demonstrated the feasibility of conducting cell mechanobiology investigations with the two novel devices, while also highlighting the experimental capabilities of non-uniform mechanical environments for these types of studies. Such capabilities include robust data collection for developing mechanobiological dose-response curves, signal threshold identification, and potential spatial targeting for drug delivery.

Full Text

Duke Authors

Cited Authors

  • Richardson, WJ; van der Voort, DD; Wilson, E; Moore, JE

Published Date

  • September 2013

Published In

Volume / Issue

  • 10 / 3

Start / End Page

  • 245 - 265

PubMed ID

  • 24396979

Pubmed Central ID

  • 24396979

Electronic International Standard Serial Number (EISSN)

  • 1556-5300

International Standard Serial Number (ISSN)

  • 1556-5297

Language

  • eng