Test-retest variability of multifocal electroretinography in normal volunteers and short-term variability in hydroxychloroquine users


Journal Article

Purpose: To determine measurement variability of N1P1 amplitudes and the R1/R2 ratio in normal subjects and hydroxychloroquine users without retinopathy. Design: Retrospective, observational study. Subjects: Normal subjects (n=21) and 44 patients taking hydroxychloroquine (n=44) without retinopathy. Methods: Multifocal electroretinography (mfERG) was performed twice in one session in the 21 normal subjects and twice within 1 year in the hydroxychloroquine users, during which time no clinical change in macular status occurred. Main outcome measures: N1P1 amplitudes of rings R1-R5, the R1/R2 ratio, and coefficients of repeatability (COR) for these measurements. Results: Values for N1P1 amplitudes in hydroxychloroquine users were reduced compared with normal subjects by the known effect of age, but R1/R2 was not affected by age. The COR for R1-R5 ranged from 43% to 52% for normal subjects and from 43% to 59% for hydroxychloroquine users; for R1/R2 the COR was 29% in normal subjects and 45% in hydroxychloroquine users. Conclusion: mfERG measurements show high test-retest variability, limiting the ability of a single mfERG test to influence a decision to stop hydroxychloroquine; corroborative evidence with a different ancillary test is recommended in a suspicious case. © 2014 Browning and Lee.

Full Text

Duke Authors

Cited Authors

  • Browning, DJ; Lee, C

Published Date

  • August 7, 2014

Published In

Volume / Issue

  • 8 /

Start / End Page

  • 1467 - 1473

Electronic International Standard Serial Number (EISSN)

  • 1177-5483

International Standard Serial Number (ISSN)

  • 1177-5467

Digital Object Identifier (DOI)

  • 10.2147/OPTH.S66528

Citation Source

  • Scopus