Impaired granulocyte superoxide production and prolongation of the respiratory burst due to a low-affinity NADPH-dependent oxidase.


Journal Article

A 7-yr-old girl with self-limited pulmonary aspergillosis was found to have a defect in granulocyte superoxide production. Her cells produced superoxide at 3% of control rates in response to phorbol myristate acetate (PMA) and opsonized zymosan. Lag times for O-2 production were normal with PMA, opsonized zymosan, and concanavalin-A stimulation. Her granulocyte membranes depolarized in response to all of these stimuli. Superoxide produced by podosomes and a particulate fraction demonstrated an enzyme activity with a normal maximal velocity but a decreased affinity for NADPH. NADH-dependent superoxide production by particles was similar with patient and control material. The duration of superoxide production was prolonged in the patient's intact granulocytes and in the particulate fractions from her cells. Bacterial killing by the patient's granulocytes was initially low, but approached control values after 90 min of incubation. These results are explained by an enzyme activity that has a decreased affinity for its substrate and a decreased rate of inactivation. Family studies indicate an autosomal recessive mode of inheritance.

Full Text

Duke Authors

Cited Authors

  • Shurin, SB; Cohen, HJ; Whitin, JC; Newburger, PE

Published Date

  • September 1, 1983

Published In

Volume / Issue

  • 62 / 3

Start / End Page

  • 564 - 571

PubMed ID

  • 6309280

Pubmed Central ID

  • 6309280

International Standard Serial Number (ISSN)

  • 0006-4971


  • eng

Conference Location

  • United States