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Expression of extracellular glutathione peroxidase in human and mouse gastrointestinal tract.

Publication ,  Journal Article
Tham, DM; Whitin, JC; Kim, KK; Zhu, SX; Cohen, HJ
Published in: Am J Physiol
December 1998

Extracellular glutathione peroxidase (EGPx) is a glycosylated selenoprotein capable of reducing hydrogen peroxide, organic hydroperoxides, free fatty acid hydroperoxides, and phosphatidylcholine hydroperoxides. We found that human large intestinal explant cultures synthesize EGPx and cellular glutathione peroxidase (CGPx) and secrete EGPx. The level of EGPx mRNA expression relative to alpha-tubulin was similar throughout the mouse gastrointestinal tract. EGPx mRNA transcripts have been localized to mature absorptive epithelial cells in human and mouse large intestine. Western blot analysis of mouse intestinal protein has demonstrated the presence of EGPx protein in the small intestine, cecum, and large intestine, with the highest protein levels found in the cecum. Immunohistochemistry studies of human large intestine and mouse small and large intestine sections demonstrated the presence of EGPx protein within mature absorptive epithelial cells. In human large intestine and mouse small intestine, EGPx protein is also present in the extracellular milieu. These results suggest a role for EGPx in protection of the intestinal tract from peroxidative damage and/or in intercellular metabolism of peroxides.

Duke Scholars

Published In

Am J Physiol

DOI

ISSN

0002-9513

Publication Date

December 1998

Volume

275

Issue

6

Start / End Page

G1463 / G1471

Location

United States

Related Subject Headings

  • Tissue Distribution
  • RNA, Messenger
  • Mice, Inbred BALB C
  • Mice
  • Intestine, Small
  • Intestine, Large
  • In Vitro Techniques
  • Humans
  • Glutathione Peroxidase
  • Extracellular Space
 

Citation

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Tham, D. M., Whitin, J. C., Kim, K. K., Zhu, S. X., & Cohen, H. J. (1998). Expression of extracellular glutathione peroxidase in human and mouse gastrointestinal tract. Am J Physiol, 275(6), G1463–G1471. https://doi.org/10.1152/ajpgi.1998.275.6.G1463
Tham, D. M., J. C. Whitin, K. K. Kim, S. X. Zhu, and H. J. Cohen. “Expression of extracellular glutathione peroxidase in human and mouse gastrointestinal tract.Am J Physiol 275, no. 6 (December 1998): G1463–71. https://doi.org/10.1152/ajpgi.1998.275.6.G1463.
Tham DM, Whitin JC, Kim KK, Zhu SX, Cohen HJ. Expression of extracellular glutathione peroxidase in human and mouse gastrointestinal tract. Am J Physiol. 1998 Dec;275(6):G1463–71.
Tham, D. M., et al. “Expression of extracellular glutathione peroxidase in human and mouse gastrointestinal tract.Am J Physiol, vol. 275, no. 6, Dec. 1998, pp. G1463–71. Pubmed, doi:10.1152/ajpgi.1998.275.6.G1463.
Tham DM, Whitin JC, Kim KK, Zhu SX, Cohen HJ. Expression of extracellular glutathione peroxidase in human and mouse gastrointestinal tract. Am J Physiol. 1998 Dec;275(6):G1463–G1471.

Published In

Am J Physiol

DOI

ISSN

0002-9513

Publication Date

December 1998

Volume

275

Issue

6

Start / End Page

G1463 / G1471

Location

United States

Related Subject Headings

  • Tissue Distribution
  • RNA, Messenger
  • Mice, Inbred BALB C
  • Mice
  • Intestine, Small
  • Intestine, Large
  • In Vitro Techniques
  • Humans
  • Glutathione Peroxidase
  • Extracellular Space