Epithelioid osteosarcoma of bone. Immunocytochemical evidence suggesting divergent epithelial and mesenchymal differentiation in a primary osseous neoplasm.


Journal Article

BACKGROUND:The combination of a primary osteosarcoma of bone with a second carcinomatous cell type has been recognized, although immunohistochemical studies currently have not been performed in an attempt to understand the histogenesis of such a tumor. METHODS:In this report, the authors performed immunohistochemical studies on a primary osseous carcinosarcoma. Using a biotin-streptavidin peroxidase conjugate technique, the expression of keratin, epithelial membrane antigen, and vimentin was analyzed. RESULTS:The epithelial cells expressed cytokeratin and epithelial membrane antigen but did not express vimentin. The mesenchymal cells strongly expressed vimentin, and only rare cells expressed cytokeratin. CONCLUSIONS:The clinical, morphologic, and immunophenotypic data in this instance strongly suggest divergent differentiation of a primitive multipotential uncommitted stem cell in a primary osseous tumor.

Full Text

Duke Authors

Cited Authors

  • Kramer, K; Hicks, DG; Palis, J; Rosier, RN; Oppenheimer, J; Fallon, MD; Cohen, HJ

Published Date

  • May 1993

Published In

Volume / Issue

  • 71 / 10

Start / End Page

  • 2977 - 2982

PubMed ID

  • 7683966

Pubmed Central ID

  • 7683966

Electronic International Standard Serial Number (EISSN)

  • 1097-0142

International Standard Serial Number (ISSN)

  • 0008-543X

Digital Object Identifier (DOI)

  • 10.1002/1097-0142(19930515)71:10<2977::aid-cncr2820711015>3.0.co;2-u


  • eng