Microfluidic Device for Coupling Capillary Electrophoresis and Matrix-Assisted Laser Desorption Ionization-Mass Spectrometry


Journal Article

We have designed and fabricated a polydimethylsiloxane (PDMS) microfluidic device for coupling capillary electrophoresis (CE) and matrix-assisted laser desorption ionization-mass spectrometry (MALDI-MS). The coupling is advantageous in biological research because CE has the power of separating analytes in a sample based on mobility difference and MALDI-MS provides accurate and sensitive mass analysis of the analytes. The goal is realized by fractionating the separated analytes inside the microfluidic device and pushing the analyte fractions into open reservoirs. Each analyte fraction is then mixed with a matrix solution and deposited on a MALDI target for MALDI-MS. Therefore, a two-step analysis of analytes in the form of CE-MALDI-MS is achieved by using the microfluidic device. © 2009, Society for Laboratory Automation and Screening. All rights reserved.

Full Text

Duke Authors

Cited Authors

  • Luo, Y; Xu, S; Schilling, JW; Lau, KH; Whitin, JC; Yu, TTS; Cohen, HJ

Published Date

  • January 1, 2009

Published In

Volume / Issue

  • 14 / 5

Start / End Page

  • 252 - 261

Electronic International Standard Serial Number (EISSN)

  • 1540-2452

International Standard Serial Number (ISSN)

  • 2211-0682

Digital Object Identifier (DOI)

  • 10.1016/j.jala.2009.02.005

Citation Source

  • Scopus