Molecular detection and analysis of spotted fever group Rickettsia in patients with fever and rash at a tertiary care centre in Tamil Nadu, India.

Published

Journal Article

BACKGROUND: Detection of specific targets by PCR is used to confirm a diagnosis of spotted fever, but serological tests are still widely used. In this prospective study, nested PCR was performed on skin biopsy specimens to confirm the diagnosis of spotted fever. METHODS: In 58 clinically suspected cases of spotted fever, nested PCR, to detect gltA, 17 kDa lipoprotein antigen gene (17 kDa), ompA and ompB, from skin biopsy of the rash was performed. Sequencing was carried on amplicons representing the four targets to confirm specificity of amplification. This was followed by phylogenetic analysis using MEGA version 4.0 software. RESULTS: The gltA, 17 kDa, ompA, and ompB genes were detected from skin biopsy specimens in 38, 23, 27, and 22 individuals. Sequence analysis revealed that the gltA, 17 kDa, ompA, and ompB sequences belonged to spotted fever group (SFG) rickettsia. Of the six partial ompA gene sequences, only one was dissimilar to the previously reported 'Candidatus Rickettsia kellyi'. CONCLUSION: Further evidence indicates that SFG rickettsiae resembling 'Candidatus Rickettsia kellyi' cause fever and rash in southern India. More detailed phylogenetic analysis following isolation of rickettsia in culture is required for providing irrefutable proof for the occurrence of novel spotted fever rickettsiae in this region.

Full Text

Duke Authors

Cited Authors

  • Prakash, JAJ; Sohan Lal, T; Rosemol, V; Verghese, VP; Pulimood, SA; Reller, M; Dumler, JS

Published Date

  • March 2012

Published In

Volume / Issue

  • 106 / 1

Start / End Page

  • 40 - 45

PubMed ID

  • 22595273

Pubmed Central ID

  • 22595273

Electronic International Standard Serial Number (EISSN)

  • 2047-7732

Digital Object Identifier (DOI)

  • 10.1179/2047773212Y.0000000001

Language

  • eng

Conference Location

  • England