Skip to main content
Journal cover image

TiO2 Nanoparticle-Induced Oxidation of the Plasma Membrane: Importance of the Protein Corona.

Publication ,  Journal Article
Runa, S; Lakadamyali, M; Kemp, ML; Payne, CK
Published in: The journal of physical chemistry. B
September 2017

Titanium dioxide (TiO2) nanoparticles, used as pigments and photocatalysts, are widely present in modern society. Inhalation or ingestion of these nanoparticles can lead to cellular-level interactions. We examined the very first step in this cellular interaction, the effect of TiO2 nanoparticles on the lipids of the plasma membrane. Within 12 h of TiO2 nanoparticle exposure, the lipids of the plasma membrane were oxidized, determined with a malondialdehyde assay. Lipid peroxidation was inhibited by surface passivation of the TiO2 nanoparticles, incubation with an antioxidant (Trolox), and the presence of serum proteins in solution. Subsequent experiments determined that serum proteins adsorbed on the surface of the TiO2 nanoparticles, forming a protein corona, inhibit lipid peroxidation. Super-resolution fluorescence microscopy showed that these serum proteins were clustered on the nanoparticle surface. These protein clusters slow lipid peroxidation, but by 24 h, the level of lipid peroxidation is similar, independent of the protein corona or free serum proteins. Additionally, over 24 h, this corona of proteins was displaced from the nanoparticle surface by free proteins in solution. Overall, these experiments provide the first mechanistic investigation of plasma membrane oxidation by TiO2 nanoparticles, in the absence of UV light and as a function of the protein corona, approximating a physiological environment.

Duke Scholars

Altmetric Attention Stats
Dimensions Citation Stats

Published In

The journal of physical chemistry. B

DOI

EISSN

1520-5207

ISSN

1520-6106

Publication Date

September 2017

Volume

121

Issue

37

Start / End Page

8619 / 8625

Related Subject Headings

  • Tumor Cells, Cultured
  • Titanium
  • Surface Properties
  • Serum Albumin, Bovine
  • Particle Size
  • Oxidation-Reduction
  • Nanoparticles
  • Microscopy, Fluorescence
  • Lipid Peroxidation
  • Humans
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Runa, S., Lakadamyali, M., Kemp, M. L., & Payne, C. K. (2017). TiO2 Nanoparticle-Induced Oxidation of the Plasma Membrane: Importance of the Protein Corona. The Journal of Physical Chemistry. B, 121(37), 8619–8625. https://doi.org/10.1021/acs.jpcb.7b04208
Runa, Sabiha, Melike Lakadamyali, Melissa L. Kemp, and Christine K. Payne. “TiO2 Nanoparticle-Induced Oxidation of the Plasma Membrane: Importance of the Protein Corona.The Journal of Physical Chemistry. B 121, no. 37 (September 2017): 8619–25. https://doi.org/10.1021/acs.jpcb.7b04208.
Runa S, Lakadamyali M, Kemp ML, Payne CK. TiO2 Nanoparticle-Induced Oxidation of the Plasma Membrane: Importance of the Protein Corona. The journal of physical chemistry B. 2017 Sep;121(37):8619–25.
Runa, Sabiha, et al. “TiO2 Nanoparticle-Induced Oxidation of the Plasma Membrane: Importance of the Protein Corona.The Journal of Physical Chemistry. B, vol. 121, no. 37, Sept. 2017, pp. 8619–25. Epmc, doi:10.1021/acs.jpcb.7b04208.
Runa S, Lakadamyali M, Kemp ML, Payne CK. TiO2 Nanoparticle-Induced Oxidation of the Plasma Membrane: Importance of the Protein Corona. The journal of physical chemistry B. 2017 Sep;121(37):8619–8625.
Journal cover image

Published In

The journal of physical chemistry. B

DOI

EISSN

1520-5207

ISSN

1520-6106

Publication Date

September 2017

Volume

121

Issue

37

Start / End Page

8619 / 8625

Related Subject Headings

  • Tumor Cells, Cultured
  • Titanium
  • Surface Properties
  • Serum Albumin, Bovine
  • Particle Size
  • Oxidation-Reduction
  • Nanoparticles
  • Microscopy, Fluorescence
  • Lipid Peroxidation
  • Humans