A high mitochondrial transport rate characterizes CNS neurons with high axonal regeneration capacity.

Published

Journal Article

Improving axonal transport in the injured and diseased central nervous system has been proposed as a promising strategy to improve neuronal repair. However, the contribution of each cargo to the repair mechanism is unknown. DRG neurons globally increase axonal transport during regeneration. Because the transport of specific cargos after axonal insult has not been examined systematically in a model of enhanced regenerative capacity, it is unknown whether the transport of all cargos would be modulated equally in injured central nervous system neurons. Here, using a microfluidic culture system we compared neurons co-deleted for PTEN and SOCS3, an established model of high axonal regeneration capacity, to control neurons. We measured the axonal transport of three cargos (mitochondria, synaptic vesicles and late endosomes) in regenerating axons and found that the transport of mitochondria, but not the other cargos, was increased in PTEN/SOCS3 co-deleted axons relative to controls. The results reported here suggest a pivotal role for this organelle during axonal regeneration.

Full Text

Duke Authors

Cited Authors

  • Cartoni, R; Pekkurnaz, G; Wang, C; Schwarz, TL; He, Z

Published Date

  • January 2017

Published In

Volume / Issue

  • 12 / 9

Start / End Page

  • e0184672 -

PubMed ID

  • 28926622

Pubmed Central ID

  • 28926622

Electronic International Standard Serial Number (EISSN)

  • 1932-6203

International Standard Serial Number (ISSN)

  • 1932-6203

Digital Object Identifier (DOI)

  • 10.1371/journal.pone.0184672

Language

  • eng