Intestinal Alkaline Phosphatase Attenuates Alcohol-Induced Hepatosteatosis in Mice.

Journal Article

Background and aims

Bacterially derived factors from the gut play a major role in the activation of inflammatory pathways in the liver and in the pathogenesis of alcoholic liver disease. The intestinal brush-border enzyme intestinal alkaline phosphatase (IAP) detoxifies a variety of bacterial pro-inflammatory factors and also functions to preserve gut barrier function. The aim of this study was to investigate whether oral IAP supplementation could protect against alcohol-induced liver disease.


Mice underwent acute binge or chronic ethanol exposure to induce alcoholic liver injury and steatosis ± IAP supplementation. Liver tissue was assessed for biochemical, inflammatory, and histopathological changes. An ex vivo co-culture system was used to examine the effects of alcohol and IAP treatment in regard to the activation of hepatic stellate cells and their role in the development of alcoholic liver disease.


Pretreatment with IAP resulted in significantly lower serum alanine aminotransferase compared to the ethanol alone group in the acute binge model. IAP treatment attenuated the development of alcohol-induced fatty liver, lowered hepatic pro-inflammatory cytokine and serum LPS levels, and prevented alcohol-induced gut barrier dysfunction. Finally, IAP ameliorated the activation of hepatic stellate cells and prevented their lipogenic effect on hepatocytes.


IAP treatment protected mice from alcohol-induced hepatotoxicity and steatosis. Oral IAP supplementation could represent a novel therapy to prevent alcoholic-related liver disease in humans.

Full Text

Duke Authors

Cited Authors

  • Hamarneh, SR; Kim, B-M; Kaliannan, K; Morrison, SA; Tantillo, TJ; Tao, Q; Mohamed, MMR; Ramirez, JM; Karas, A; Liu, W; Hu, D; Teshager, A; Gul, SS; Economopoulos, KP; Bhan, AK; Malo, MS; Choi, MY; Hodin, RA

Published Date

  • August 2017

Published In

Volume / Issue

  • 62 / 8

Start / End Page

  • 2021 - 2034

PubMed ID

  • 28424943

Pubmed Central ID

  • 28424943

Electronic International Standard Serial Number (EISSN)

  • 1573-2568

International Standard Serial Number (ISSN)

  • 0163-2116

Digital Object Identifier (DOI)

  • 10.1007/s10620-017-4576-0


  • eng