Cyclin D/CDK4/6 activity controls G1 length in mammalian cells.

Published online

Journal Article

The length of the G1 phase in the cell cycle shows significant variability in different cell types and tissue types. To gain insights into the control of G1 length, we generated an E2F activity reporter that captures free E2F activity after dissociation from Rb sequestration and followed its kinetics of activation at the single-cell level, in real time. Our results demonstrate that its activity is precisely coordinated with S phase progression. Quantitative analysis indicates that there is a pre-S phase delay between E2F transcriptional dynamic and activity dynamics. This delay is variable among different cell types and is strongly modulated by the cyclin D/CDK4/6 complex activity through Rb phosphorylation. Our findings suggest that the main function of this complex is to regulate the appropriate timing of G1 length.

Full Text

Duke Authors

Cited Authors

  • Dong, P; Zhang, C; Parker, B-T; You, L; Mathey-Prevot, B

Published Date

  • 2018

Published In

Volume / Issue

  • 13 / 1

Start / End Page

  • e0185637 -

PubMed ID

  • 29309421

Pubmed Central ID

  • 29309421

Electronic International Standard Serial Number (EISSN)

  • 1932-6203

Digital Object Identifier (DOI)

  • 10.1371/journal.pone.0185637

Language

  • eng

Conference Location

  • United States