Functional interrogation and mining of natively paired human VH:VL antibody repertoires.
Journal Article (Journal Article)
We present a technology to screen millions of B cells for natively paired human antibody repertoires. Libraries of natively paired, variable region heavy and light (VH:VL) amplicons are expressed in a yeast display platform that is optimized for human Fab surface expression. Using our method we identify HIV-1 broadly neutralizing antibodies (bNAbs) from an HIV-1 slow progressor and high-affinity neutralizing antibodies against Ebola virus glycoprotein and influenza hemagglutinin.
Full Text
Duke Authors
Cited Authors
- Wang, B; DeKosky, BJ; Timm, MR; Lee, J; Normandin, E; Misasi, J; Kong, R; McDaniel, JR; Delidakis, G; Leigh, KE; Niezold, T; Choi, CW; Viox, EG; Fahad, A; Cagigi, A; Ploquin, A; Leung, K; Yang, ES; Kong, W-P; Voss, WN; Schmidt, AG; Moody, MA; Ambrozak, DR; Henry, AR; Laboune, F; Ledgerwood, JE; Graham, BS; Connors, M; Douek, DC; Sullivan, NJ; Ellington, AD; Mascola, JR; Georgiou, G
Published Date
- February 2018
Published In
Volume / Issue
- 36 / 2
Start / End Page
- 152 - 155
PubMed ID
- 29309060
Pubmed Central ID
- PMC5801115
Electronic International Standard Serial Number (EISSN)
- 1546-1696
Digital Object Identifier (DOI)
- 10.1038/nbt.4052
Language
- eng
Conference Location
- United States