Functional interrogation and mining of natively paired human VH:VL antibody repertoires.

Journal Article (Journal Article)

We present a technology to screen millions of B cells for natively paired human antibody repertoires. Libraries of natively paired, variable region heavy and light (VH:VL) amplicons are expressed in a yeast display platform that is optimized for human Fab surface expression. Using our method we identify HIV-1 broadly neutralizing antibodies (bNAbs) from an HIV-1 slow progressor and high-affinity neutralizing antibodies against Ebola virus glycoprotein and influenza hemagglutinin.

Full Text

Duke Authors

Cited Authors

  • Wang, B; DeKosky, BJ; Timm, MR; Lee, J; Normandin, E; Misasi, J; Kong, R; McDaniel, JR; Delidakis, G; Leigh, KE; Niezold, T; Choi, CW; Viox, EG; Fahad, A; Cagigi, A; Ploquin, A; Leung, K; Yang, ES; Kong, W-P; Voss, WN; Schmidt, AG; Moody, MA; Ambrozak, DR; Henry, AR; Laboune, F; Ledgerwood, JE; Graham, BS; Connors, M; Douek, DC; Sullivan, NJ; Ellington, AD; Mascola, JR; Georgiou, G

Published Date

  • February 2018

Published In

Volume / Issue

  • 36 / 2

Start / End Page

  • 152 - 155

PubMed ID

  • 29309060

Pubmed Central ID

  • PMC5801115

Electronic International Standard Serial Number (EISSN)

  • 1546-1696

Digital Object Identifier (DOI)

  • 10.1038/nbt.4052

Language

  • eng

Conference Location

  • United States