Fetal gene transfer by transuterine injection of cationic liposome-DNA complexes.


Journal Article

In utero injection of cationic liposome-DNA complexes (CLDCs) containing chloramphenicol acetyltransferase, beta-galactosidase (beta-gal), or human granulocyte colony-stimulating factor (hG-CSF) expression plasmids produced high-level gene expression in fetal rats. Tissues adjacent to the injection site exhibited the highest levels of gene expression. Chloramphenicol acetyltransferase expression persisted for at least 14 days and was reexpressed following postnatal reinjection of CLDCs. Intraperitoneal administration of the hG-CSF gene produced high serum hG-CSF levels. X-gal staining demonstrated widespread beta-gal expression in multiple fetal tissues and cell types. No toxic or inflammatory responses were observed, nor was there evidence of fetal-maternal or maternal-fetal gene transfer, suggesting that CLDCs may provide a useful alternative to viral vectors for in utero gene transfer.

Full Text

Cited Authors

  • Gaensler, KM; Tu, G; Bruch, S; Liggitt, D; Lipshutz, GS; Metkus, A; Harrison, M; Heath, TD; Debs, RJ

Published Date

  • December 1999

Published In

Volume / Issue

  • 17 / 12

Start / End Page

  • 1188 - 1192

PubMed ID

  • 10585716

Pubmed Central ID

  • 10585716

Electronic International Standard Serial Number (EISSN)

  • 1546-1696

International Standard Serial Number (ISSN)

  • 1087-0156

Digital Object Identifier (DOI)

  • 10.1038/70729


  • eng