In-utero transplantation of fetal liver haemopoietic stem cells in monkeys.

Published

Journal Article

To evaluate the potential of in-utero transplantation of fetal haemopoietic stem cells (HSCs) for permanent engraftment as a treatment of congenital haemoglobinopathies, fetal rhesus monkeys were transplanted with HSCs derived from fetal livers. Five pregnant monkeys (60-62 days' gestation) were given an in-utero intraperitoneal injection of fetal liver cells (10(8)-10(9) cells/kg estimated fetal recipient body weight) derived from opposite sex donors at 59-68 days' gestation. Engraftment was confirmed by karyotype analysis of peripheral blood leucocytes and bone marrow; cells of donor sex were found among the recipient cells. Donor cell engraftment was apparent in four of five in-utero HSC transplant recipients at birth. Engraftment involved lymphoid (2.9-8.0% donor cells), erythroid (5.3-12.5%), and myeloid (8.5-15.4%) lineages and has persisted for up to 2 years without evidence of graft-versus-host disease.

Full Text

Cited Authors

  • Harrison, MR; Slotnick, RN; Crombleholme, TM; Golbus, MS; Tarantal, AF; Zanjani, ED

Published Date

  • December 1989

Published In

Volume / Issue

  • 2 / 8677

Start / End Page

  • 1425 - 1427

PubMed ID

  • 2574363

Pubmed Central ID

  • 2574363

Electronic International Standard Serial Number (EISSN)

  • 1474-547X

International Standard Serial Number (ISSN)

  • 0140-6736

Digital Object Identifier (DOI)

  • 10.1016/s0140-6736(89)92036-9

Language

  • eng