Identification of candidate prostate cancer biomarkers in prostate needle biopsy specimens using proteomic analysis.

Published

Journal Article

Although serum prostate specific antigen (PSA) is a well-established diagnostic tool for prostate cancer (PCa) detection, the definitive diagnosis of PCa is based on the information contained in prostate needle biopsy (PNBX) specimens. To define the proteomic features of PNBX specimens to identify candidate biomarkers for PCa, PNBX specimens from patients with PCa or benign prostatic hyperplasia (BPH) were subjected to comparative proteomic analysis. 2-DE revealed that 52 protein spots exhibited statistically significantly changes among PCa and BPH groups. Interesting spots were identified by MALDI-TOF-MS/MS. The 2 most notable groups of proteins identified included latent androgen receptor coregulators [FLNA(7-15) and FKBP4] and enzymes involved in mitochondrial fatty acid beta-oxidation (DCI and ECHS1). An imbalance in the expression of peroxiredoxin subtypes was noted in PCa specimens. Furthermore, different post-translationally modified isoforms of HSP27 and HSP70.1 were identified. Importantly, changes in FLNA(7-15), FKBP4, and PRDX4 expression were confirmed by immunoblot analyses. Our results suggest that a proteomics-based approach is useful for developing a more complete picture of the protein profile of PNBX specimen. The proteins identified by this approach may be useful molecular targets for PCa diagnostics and therapeutics.

Full Text

Cited Authors

  • Lin, J-F; Xu, J; Tian, H-Y; Gao, X; Chen, Q-X; Gu, Q; Xu, G-J; Song, J-D; Zhao, F-K

Published Date

  • December 2007

Published In

Volume / Issue

  • 121 / 12

Start / End Page

  • 2596 - 2605

PubMed ID

  • 17722004

Pubmed Central ID

  • 17722004

Electronic International Standard Serial Number (EISSN)

  • 1097-0215

International Standard Serial Number (ISSN)

  • 0020-7136

Digital Object Identifier (DOI)

  • 10.1002/ijc.23016

Language

  • eng