Quantum-dot-decorated robust transductable bioluminescent nanocapsules.


Journal Article

Bioluminescence, due to its high sensitivity, has been exploited in various analytical and imaging applications. In this work, we report a highly stable, cell-transductable, and wavelength-tunable bioluminescence system achieved with an elegant and simple design. Using aqueous in situ polymerization on a bioluminescent enzyme anchored with polymerizable vinyl groups, we obtained nanosized core-shell nanocapsules with the enzyme as the core and a cross-linked thin polymer net as the shell. These nanocapsules possess greatly enhanced stability, retained bioactivity, and a readily engineered surface. In particular, by incorporating polymerizable amines in the polymerization, we endowed the nanocapsules with efficient cell-transduction and sufficient conjugation sites for follow-up modification. Following in situ polymerization, decorating the polymer shell with fluorescent quantum dots allowed us to access a continuous tunable wavelength, which extends the application of such bioluminescent nanocapsules, especially in deep tissue. In addition, the unique core-shell structure and adequate conjugation sites on surface enabled us to maximize the BRET efficiency by adjusting the QD/enzyme conjugation ratio.

Full Text

Duke Authors

Cited Authors

  • Du, J; Yu, C; Pan, D; Li, J; Chen, W; Yan, M; Segura, T; Lu, Y

Published Date

  • September 2010

Published In

Volume / Issue

  • 132 / 37

Start / End Page

  • 12780 - 12781

PubMed ID

  • 20795619

Pubmed Central ID

  • 20795619

Electronic International Standard Serial Number (EISSN)

  • 1520-5126

International Standard Serial Number (ISSN)

  • 0002-7863

Digital Object Identifier (DOI)

  • 10.1021/ja104299t


  • eng