Incorporation of active DNA/cationic polymer polyplexes into hydrogel scaffolds.

Published

Journal Article

The effective and sustained delivery of DNA and siRNAs locally would increase the applicability of gene therapy in tissue regeneration and cancer therapy. One promising approach is to use hydrogel scaffolds to encapsulate and deliver nucleotides in the form of nanoparticles to the disease sites. However, this approach is currently limited by the inability to load concentrated and active gene delivery nanoparticles into the hydrogels due to the severe nanoparticle aggregation during the loading process. Here, we present a process to load concentrated and un-aggregated non-viral gene delivery nanoparticles, using DNA/polyethylene imine (PEI) polyplexes as an example, into neutral polyethylene glycol (PEG), negatively charged hyaluronic acid (HA) and protein fibrin hydrogels crosslinked through various chemistries. The encapsulated polyplexes are highly active both in vitro and in vivo. We believe this process will significantly advance the applications of hydrogel scaffold mediated non-viral gene delivery in tissue regeneration and cancer therapy.

Full Text

Duke Authors

Cited Authors

  • Lei, Y; Huang, S; Sharif-Kashani, P; Chen, Y; Kavehpour, P; Segura, T

Published Date

  • December 2010

Published In

Volume / Issue

  • 31 / 34

Start / End Page

  • 9106 - 9116

PubMed ID

  • 20822811

Pubmed Central ID

  • 20822811

Electronic International Standard Serial Number (EISSN)

  • 1878-5905

International Standard Serial Number (ISSN)

  • 0142-9612

Digital Object Identifier (DOI)

  • 10.1016/j.biomaterials.2010.08.016

Language

  • eng