Two and three-dimensional gene transfer from enzymatically degradable hydrogel scaffolds.

Published

Journal Article

The ability to genetically modify mesenchymal stem cells (MSCs) seeded inside synthetic hydrogel scaffolds would offer an alternative approach to guide MSC differentiation. In this report, we explored gene transfer to MSCs seeded on top or inside matrix metalloproteinase (MMP) degradable hydrogels that were loaded with DNA/poly(ethylene imine) (PEI) polyplexes. DNA/PEI polyplexes were encapsulated inside poly(ethylene glycol) (PEG) hydrogels crosslinked with MMP degradable peptides via Michael Addition chemistry. Gene transfer was visualized and quantified through using a vector encoding for green fluorescent protein and luciferase. We found that gene transfer to MSCs was possible for cells seeded both in two and three dimensions. The amount of luciferase expression was similar for cells seeded in two and three dimensions even though the number of cells in three dimensions is significantly higher, indicating that gene transfer to cells seeded in two dimensions is more efficient than for cells seeded in three dimensions. The use of hydrogel scaffolds that allow cellular infiltration to deliver DNA may result in long-lasting signals in vivo, which are essential for the regeneration of functional tissues.

Full Text

Duke Authors

Cited Authors

  • Lei, Y; Ng, QKT; Segura, T

Published Date

  • September 2010

Published In

Volume / Issue

  • 73 / 9

Start / End Page

  • 910 - 917

PubMed ID

  • 20232458

Pubmed Central ID

  • 20232458

Electronic International Standard Serial Number (EISSN)

  • 1097-0029

International Standard Serial Number (ISSN)

  • 1059-910X

Digital Object Identifier (DOI)

  • 10.1002/jemt.20840

Language

  • eng