Transcriptome regulation and chromatin occupancy by E2F3 and MYC in mice.

Journal Article (Journal Article)

E2F3 and MYC are transcription factors that control cellular proliferation. To study their mechanism of action in the context of a regenerating tissue, we isolated both proliferating (crypts) and non-dividing (villi) cells from wild-type and Rb depleted small intestines of mice and performed ChIP-exo-seq (chromatin immunoprecipitation combined with lambda exonuclease digestion followed by high-throughput sequencing). The genome-wide chromatin occupancy of E2F3 and MYC was determined by mapping sequence reads to the genome and predicting preferred binding sites (peaks). Binding sites could be accurately identified within small regions of only 24 bp-28 bp long, highlighting the precision to which binding peaks can be identified by ChIP-exo-seq. Forty randomly selected E2F3- and MYC-specific binding sites were validated by ChIP-PCR. In addition, we also presented gene expression data sets from wild type, Rb-, E2f3- and Myc-depleted crypts and villi within this manuscript. These represent comprehensive and validated datasets that can be integrated to identify putative direct targets of E2F3 and MYC involved in the control of cellular proliferation in normal and Rb-deficient small intestines.

Full Text

Duke Authors

Cited Authors

  • Tang, X; Liu, H; Srivastava, A; Pécot, T; Chen, Z; Wang, Q; Huang, K; Sáenz-Robles, MT; Cantalupo, P; Pipas, J; Leone, G

Published Date

  • February 16, 2016

Published In

Volume / Issue

  • 3 /

Start / End Page

  • 160008 -

PubMed ID

  • 26881867

Pubmed Central ID

  • PMC4755127

Electronic International Standard Serial Number (EISSN)

  • 2052-4463

Digital Object Identifier (DOI)

  • 10.1038/sdata.2016.8

Language

  • eng

Conference Location

  • England