Flow Cytometric Identification of Tumor-Infiltrating Lymphocytes from Glioblastoma.

Journal Article (Journal Article)

We describe an isolation method of tumor-infiltrating lymphocytes (TILs) from glioblastoma tumors for the purpose of analysis by flow cytometry. This protocol is unique from many others in that the use of a selective lymphocyte isolation procedure, such as a Ficoll or Percoll gradient, is not used. We find that staining of TILs and analysis by flow cytometry is not affected by the presence of heterogeneous populations, while other selective isolation procedures can significantly decrease lymphocyte yield from already rare populations.

Full Text

Duke Authors

Cited Authors

  • Woroniecka, K; Chongsathidkiet, P; Elsamadicy, A; Farber, H; Cui, X; Fecci, PE

Published Date

  • 2018

Published In

Volume / Issue

  • 1741 /

Start / End Page

  • 221 - 226

PubMed ID

  • 29392704

Pubmed Central ID

  • PMC6825407

Electronic International Standard Serial Number (EISSN)

  • 1940-6029

Digital Object Identifier (DOI)

  • 10.1007/978-1-4939-7659-1_18

Language

  • eng

Conference Location

  • United States