Proteinase activation: a mechanism for cellular dyshesion in pemphigus.


Journal Article

An in vitro model system using cultured newborn epidermal cells was employed to investigate the binding of pemphigus autoantibody and subsequent loss of adhesion between epidermal cells. Pemphigus antibodies bound to both mouse and human cultured epidermal cells. Incubation of cultured newborn mouse epidermal cells with pemphigus antibody followed by gentle agitation induced loss of adhesion between the epidermal cells and the plastic culture dish. Release of viable epidermal cells from the dish was inhibited by the proteinase inhibitors, soybean trypsin inhibitor and alpha 2-macroglobulin. These observations suggest that pemphigus antibody induces viable epidermal cells to activate cellular proteinases which then degrade the glycocalyx and cause cellular dyshesion and acantholysis.

Full Text

Duke Authors

Cited Authors

  • Singer, KH; Sawka, NJ; Samowitz, HR; Lazarus, GS

Published Date

  • May 1, 1980

Published In

Volume / Issue

  • 74 / 5

Start / End Page

  • 363 - 367

PubMed ID

  • 6993576

Pubmed Central ID

  • 6993576

International Standard Serial Number (ISSN)

  • 0022-202X

Digital Object Identifier (DOI)

  • 10.1111/1523-1747.ep12543780


  • eng

Conference Location

  • United States