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Silk-Encapsulated Plasmonic Biochips with Enhanced Thermal Stability.

Publication ,  Journal Article
Wang, C; Luan, J; Tadepalli, S; Liu, K-K; Morrissey, JJ; Kharasch, ED; Naik, RR; Singamaneni, S
Published in: ACS Appl Mater Interfaces
October 12, 2016

Because of their high sensitivity, cost-efficiency, and great potential as point-of-care biodiagnostic devices, plasmonic biosensors based on localized surface plasmon resonance have gained immense attention. However, most plasmonic biosensors and conventional bioassays rely on natural antibodies, which are susceptible to elevated temperatures and nonaqueous media. Hence, an expensive and cumbersome "cold chain" system is necessary to preserve the labile antibodies by maintaining optimal cold temperatures during transport, storage, and handling. Herein, we introduce a facile approach to preserve the antibody activity on a biosensor surface even at elevated temperatures. We show that silk fibroin film could be used as a protective layer to preserve the activity of a model antibody (Rabbit IgG) and cardiac troponin antibody at both room temperature and 40 °C over several days. Furthermore, a simple aqueous rinsing process restores the biofunctionality of the biosensor. This energy-efficient and environmentally friendly method represents a novel approach to eliminate the cold chain and temperature-controlled packing of diagnostic reagents and materials, thereby extending the capability of antibody-based biosensors to different resource-limited circumstances such as developing countries, an ambulance, an intensive care unit emergency room, and battlefield.

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Published In

ACS Appl Mater Interfaces

DOI

EISSN

1944-8252

Publication Date

October 12, 2016

Volume

8

Issue

40

Start / End Page

26493 / 26500

Location

United States

Related Subject Headings

  • Temperature
  • Surface Plasmon Resonance
  • Silk
  • Rabbits
  • Nanotubes
  • Nanoscience & Nanotechnology
  • Gold
  • Biosensing Techniques
  • Animals
  • 51 Physical sciences
 

Citation

APA
Chicago
ICMJE
MLA
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Wang, C., Luan, J., Tadepalli, S., Liu, K.-K., Morrissey, J. J., Kharasch, E. D., … Singamaneni, S. (2016). Silk-Encapsulated Plasmonic Biochips with Enhanced Thermal Stability. ACS Appl Mater Interfaces, 8(40), 26493–26500. https://doi.org/10.1021/acsami.6b07362
Wang, Congzhou, Jingyi Luan, Sirimuvva Tadepalli, Keng-Ku Liu, Jeremiah J. Morrissey, Evan D. Kharasch, Rajesh R. Naik, and Srikanth Singamaneni. “Silk-Encapsulated Plasmonic Biochips with Enhanced Thermal Stability.ACS Appl Mater Interfaces 8, no. 40 (October 12, 2016): 26493–500. https://doi.org/10.1021/acsami.6b07362.
Wang C, Luan J, Tadepalli S, Liu K-K, Morrissey JJ, Kharasch ED, et al. Silk-Encapsulated Plasmonic Biochips with Enhanced Thermal Stability. ACS Appl Mater Interfaces. 2016 Oct 12;8(40):26493–500.
Wang, Congzhou, et al. “Silk-Encapsulated Plasmonic Biochips with Enhanced Thermal Stability.ACS Appl Mater Interfaces, vol. 8, no. 40, Oct. 2016, pp. 26493–500. Pubmed, doi:10.1021/acsami.6b07362.
Wang C, Luan J, Tadepalli S, Liu K-K, Morrissey JJ, Kharasch ED, Naik RR, Singamaneni S. Silk-Encapsulated Plasmonic Biochips with Enhanced Thermal Stability. ACS Appl Mater Interfaces. 2016 Oct 12;8(40):26493–26500.
Journal cover image

Published In

ACS Appl Mater Interfaces

DOI

EISSN

1944-8252

Publication Date

October 12, 2016

Volume

8

Issue

40

Start / End Page

26493 / 26500

Location

United States

Related Subject Headings

  • Temperature
  • Surface Plasmon Resonance
  • Silk
  • Rabbits
  • Nanotubes
  • Nanoscience & Nanotechnology
  • Gold
  • Biosensing Techniques
  • Animals
  • 51 Physical sciences