Silk-Encapsulated Plasmonic Biochips with Enhanced Thermal Stability.

Journal Article (Journal Article)

Because of their high sensitivity, cost-efficiency, and great potential as point-of-care biodiagnostic devices, plasmonic biosensors based on localized surface plasmon resonance have gained immense attention. However, most plasmonic biosensors and conventional bioassays rely on natural antibodies, which are susceptible to elevated temperatures and nonaqueous media. Hence, an expensive and cumbersome "cold chain" system is necessary to preserve the labile antibodies by maintaining optimal cold temperatures during transport, storage, and handling. Herein, we introduce a facile approach to preserve the antibody activity on a biosensor surface even at elevated temperatures. We show that silk fibroin film could be used as a protective layer to preserve the activity of a model antibody (Rabbit IgG) and cardiac troponin antibody at both room temperature and 40 °C over several days. Furthermore, a simple aqueous rinsing process restores the biofunctionality of the biosensor. This energy-efficient and environmentally friendly method represents a novel approach to eliminate the cold chain and temperature-controlled packing of diagnostic reagents and materials, thereby extending the capability of antibody-based biosensors to different resource-limited circumstances such as developing countries, an ambulance, an intensive care unit emergency room, and battlefield.

Full Text

Duke Authors

Cited Authors

  • Wang, C; Luan, J; Tadepalli, S; Liu, K-K; Morrissey, JJ; Kharasch, ED; Naik, RR; Singamaneni, S

Published Date

  • October 12, 2016

Published In

Volume / Issue

  • 8 / 40

Start / End Page

  • 26493 - 26500

PubMed ID

  • 27438127

Pubmed Central ID

  • PMC5371827

Electronic International Standard Serial Number (EISSN)

  • 1944-8252

Digital Object Identifier (DOI)

  • 10.1021/acsami.6b07362


  • eng

Conference Location

  • United States