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Peptide Functionalized Gold Nanorods for the Sensitive Detection of a Cardiac Biomarker Using Plasmonic Paper Devices.

Publication ,  Journal Article
Tadepalli, S; Kuang, Z; Jiang, Q; Liu, K-K; Fisher, MA; Morrissey, JJ; Kharasch, ED; Slocik, JM; Naik, RR; Singamaneni, S
Published in: Sci Rep
November 10, 2015

The sensitivity of localized surface plasmon resonance (LSPR) of metal nanostructures to adsorbates lends itself to a powerful class of label-free biosensors. Optical properties of plasmonic nanostructures are dependent on the geometrical features and the local dielectric environment. The exponential decay of the sensitivity from the surface of the plasmonic nanotransducer calls for the careful consideration in its design with particular attention to the size of the recognition and analyte layers. In this study, we demonstrate that short peptides as biorecognition elements (BRE) compared to larger antibodies as target capture agents offer several advantages. Using a bioplasmonic paper device (BPD), we demonstrate the selective and sensitive detection of the cardiac biomarker troponin I (cTnI). The smaller sized peptide provides higher sensitivity and a lower detection limit using a BPD. Furthermore, the excellent shelf-life and thermal stability of peptide-based LSPR sensors, which precludes the need for special storage conditions, makes it ideal for use in resource-limited settings.

Duke Scholars

Published In

Sci Rep

DOI

EISSN

2045-2322

Publication Date

November 10, 2015

Volume

5

Start / End Page

16206

Location

England

Related Subject Headings

  • Troponin I
  • Sweat
  • Surface Plasmon Resonance
  • Protein Binding
  • Peptides
  • Paper
  • Nanotubes
  • Humans
  • Gold
  • Biosensing Techniques
 

Citation

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Tadepalli, S., Kuang, Z., Jiang, Q., Liu, K.-K., Fisher, M. A., Morrissey, J. J., … Singamaneni, S. (2015). Peptide Functionalized Gold Nanorods for the Sensitive Detection of a Cardiac Biomarker Using Plasmonic Paper Devices. Sci Rep, 5, 16206. https://doi.org/10.1038/srep16206
Tadepalli, Sirimuvva, Zhifeng Kuang, Qisheng Jiang, Keng-Ku Liu, Marilee A. Fisher, Jeremiah J. Morrissey, Evan D. Kharasch, Joseph M. Slocik, Rajesh R. Naik, and Srikanth Singamaneni. “Peptide Functionalized Gold Nanorods for the Sensitive Detection of a Cardiac Biomarker Using Plasmonic Paper Devices.Sci Rep 5 (November 10, 2015): 16206. https://doi.org/10.1038/srep16206.
Tadepalli S, Kuang Z, Jiang Q, Liu K-K, Fisher MA, Morrissey JJ, et al. Peptide Functionalized Gold Nanorods for the Sensitive Detection of a Cardiac Biomarker Using Plasmonic Paper Devices. Sci Rep. 2015 Nov 10;5:16206.
Tadepalli, Sirimuvva, et al. “Peptide Functionalized Gold Nanorods for the Sensitive Detection of a Cardiac Biomarker Using Plasmonic Paper Devices.Sci Rep, vol. 5, Nov. 2015, p. 16206. Pubmed, doi:10.1038/srep16206.
Tadepalli S, Kuang Z, Jiang Q, Liu K-K, Fisher MA, Morrissey JJ, Kharasch ED, Slocik JM, Naik RR, Singamaneni S. Peptide Functionalized Gold Nanorods for the Sensitive Detection of a Cardiac Biomarker Using Plasmonic Paper Devices. Sci Rep. 2015 Nov 10;5:16206.

Published In

Sci Rep

DOI

EISSN

2045-2322

Publication Date

November 10, 2015

Volume

5

Start / End Page

16206

Location

England

Related Subject Headings

  • Troponin I
  • Sweat
  • Surface Plasmon Resonance
  • Protein Binding
  • Peptides
  • Paper
  • Nanotubes
  • Humans
  • Gold
  • Biosensing Techniques