Bioplasmonic calligraphy for multiplexed label-free biodetection.

Journal Article

Printable multi-marker biochips that enable simultaneous quantitative detection of multiple target biomarkers in point-of-care and resource-limited settings are a holy grail in the field of biodiagnostics. However, preserving the functionality of biomolecules, which are routinely employed as recognition elements, during conventional printing approaches remains challenging. In this article, we introduce a simple yet powerful approach, namely plasmonic calligraphy, for realizing multiplexed label-free bioassays. Plasmonic calligraphy involves a regular ballpoint pen filled with biofunctionalized gold nanorods as plasmonic ink for creating isolated test domains on paper substrates. Biofriendly plasmonic calligraphy approach serves as a facile method to miniaturize the test domain size to few mm(2), which significantly improves the sensitivity of the plasmonic biosensor compared to bioplasmonic paper fabricated using immersion approach. Furthermore, plasmonic calligraphy also serves as a simple and efficient means to isolate multiple test domains on a single test strip, which facilitates multiplexed biodetection and multi-marker biochips. Plasmonic calligraphy, which can be potentially automated by implementing with a robotic arm, serves as an alternate path forward to overcome the limitations of conventional ink-jet printing.

Full Text

Duke Authors

Cited Authors

  • Tian, L; Tadepalli, S; Park, SH; Liu, K-K; Morrissey, JJ; Kharasch, ED; Naik, RR; Singamaneni, S

Published Date

  • September 15, 2014

Published In

Volume / Issue

  • 59 /

Start / End Page

  • 208 - 215

PubMed ID

  • 24727607

Pubmed Central ID

  • 24727607

Electronic International Standard Serial Number (EISSN)

  • 1873-4235

Digital Object Identifier (DOI)

  • 10.1016/j.bios.2014.03.043


  • eng

Conference Location

  • England