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Bioplasmonic calligraphy for multiplexed label-free biodetection.

Publication ,  Journal Article
Tian, L; Tadepalli, S; Park, SH; Liu, K-K; Morrissey, JJ; Kharasch, ED; Naik, RR; Singamaneni, S
Published in: Biosens Bioelectron
September 15, 2014

Printable multi-marker biochips that enable simultaneous quantitative detection of multiple target biomarkers in point-of-care and resource-limited settings are a holy grail in the field of biodiagnostics. However, preserving the functionality of biomolecules, which are routinely employed as recognition elements, during conventional printing approaches remains challenging. In this article, we introduce a simple yet powerful approach, namely plasmonic calligraphy, for realizing multiplexed label-free bioassays. Plasmonic calligraphy involves a regular ballpoint pen filled with biofunctionalized gold nanorods as plasmonic ink for creating isolated test domains on paper substrates. Biofriendly plasmonic calligraphy approach serves as a facile method to miniaturize the test domain size to few mm(2), which significantly improves the sensitivity of the plasmonic biosensor compared to bioplasmonic paper fabricated using immersion approach. Furthermore, plasmonic calligraphy also serves as a simple and efficient means to isolate multiple test domains on a single test strip, which facilitates multiplexed biodetection and multi-marker biochips. Plasmonic calligraphy, which can be potentially automated by implementing with a robotic arm, serves as an alternate path forward to overcome the limitations of conventional ink-jet printing.

Duke Scholars

Published In

Biosens Bioelectron

DOI

EISSN

1873-4235

Publication Date

September 15, 2014

Volume

59

Start / End Page

208 / 215

Location

England

Related Subject Headings

  • Surface Plasmon Resonance
  • Paper
  • Nanotubes
  • Mice
  • Immunoglobulin G
  • Humans
  • Gold
  • Equipment Design
  • Bioinformatics
  • Antibodies, Immobilized
 

Citation

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MLA
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Tian, L., Tadepalli, S., Park, S. H., Liu, K.-K., Morrissey, J. J., Kharasch, E. D., … Singamaneni, S. (2014). Bioplasmonic calligraphy for multiplexed label-free biodetection. Biosens Bioelectron, 59, 208–215. https://doi.org/10.1016/j.bios.2014.03.043
Tian, Limei, Sirimuvva Tadepalli, Sang Hyun Park, Keng-Ku Liu, Jeremiah J. Morrissey, Evan D. Kharasch, Rajesh R. Naik, and Srikanth Singamaneni. “Bioplasmonic calligraphy for multiplexed label-free biodetection.Biosens Bioelectron 59 (September 15, 2014): 208–15. https://doi.org/10.1016/j.bios.2014.03.043.
Tian L, Tadepalli S, Park SH, Liu K-K, Morrissey JJ, Kharasch ED, et al. Bioplasmonic calligraphy for multiplexed label-free biodetection. Biosens Bioelectron. 2014 Sep 15;59:208–15.
Tian, Limei, et al. “Bioplasmonic calligraphy for multiplexed label-free biodetection.Biosens Bioelectron, vol. 59, Sept. 2014, pp. 208–15. Pubmed, doi:10.1016/j.bios.2014.03.043.
Tian L, Tadepalli S, Park SH, Liu K-K, Morrissey JJ, Kharasch ED, Naik RR, Singamaneni S. Bioplasmonic calligraphy for multiplexed label-free biodetection. Biosens Bioelectron. 2014 Sep 15;59:208–215.
Journal cover image

Published In

Biosens Bioelectron

DOI

EISSN

1873-4235

Publication Date

September 15, 2014

Volume

59

Start / End Page

208 / 215

Location

England

Related Subject Headings

  • Surface Plasmon Resonance
  • Paper
  • Nanotubes
  • Mice
  • Immunoglobulin G
  • Humans
  • Gold
  • Equipment Design
  • Bioinformatics
  • Antibodies, Immobilized