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Gas chromatographic-mass spectrometric analysis of alfentanil metabolites. Application to human liver microsomal alfentanil biotransformation.

Publication ,  Journal Article
Labroo, R; Kharasch, ED
Published in: J Chromatogr B Biomed Appl
October 3, 1994

The short-acting synthetic opioid alfentanil undergoes extensive biotransformation to several metabolites. A gas chromatographic-mass spectrometric assay, using selected-ion monitoring and deuterated internal standards, was developed for quantitating the predominant metabolites of alfentanil. Optimal extraction and derivatization conditions are described. The assay was applied to the analysis of metabolites formed during alfentanil metabolism in vitro by human liver microsomes. Formation of known alfentanil metabolites was confirmed, and formation of a metabolite, not previously detected in vitro, is described. The assay represents a significant improvement over existing methods of alfentanil metabolite analysis, which use HPLC and radiochemical detection.

Duke Scholars

Published In

J Chromatogr B Biomed Appl

DOI

ISSN

1572-6495

Publication Date

October 3, 1994

Volume

660

Issue

1

Start / End Page

85 / 94

Location

Netherlands

Related Subject Headings

  • Microsomes, Liver
  • In Vitro Techniques
  • Humans
  • Gas Chromatography-Mass Spectrometry
  • Biotransformation
  • Analytical Chemistry
  • Alfentanil
 

Citation

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ICMJE
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Labroo, R., & Kharasch, E. D. (1994). Gas chromatographic-mass spectrometric analysis of alfentanil metabolites. Application to human liver microsomal alfentanil biotransformation. J Chromatogr B Biomed Appl, 660(1), 85–94. https://doi.org/10.1016/0378-4347(94)00263-0
Labroo, R., and E. D. Kharasch. “Gas chromatographic-mass spectrometric analysis of alfentanil metabolites. Application to human liver microsomal alfentanil biotransformation.J Chromatogr B Biomed Appl 660, no. 1 (October 3, 1994): 85–94. https://doi.org/10.1016/0378-4347(94)00263-0.
Labroo, R., and E. D. Kharasch. “Gas chromatographic-mass spectrometric analysis of alfentanil metabolites. Application to human liver microsomal alfentanil biotransformation.J Chromatogr B Biomed Appl, vol. 660, no. 1, Oct. 1994, pp. 85–94. Pubmed, doi:10.1016/0378-4347(94)00263-0.

Published In

J Chromatogr B Biomed Appl

DOI

ISSN

1572-6495

Publication Date

October 3, 1994

Volume

660

Issue

1

Start / End Page

85 / 94

Location

Netherlands

Related Subject Headings

  • Microsomes, Liver
  • In Vitro Techniques
  • Humans
  • Gas Chromatography-Mass Spectrometry
  • Biotransformation
  • Analytical Chemistry
  • Alfentanil