Spine Patterning Is Guided by Segmentation of the Notochord Sheath.
Journal Article (Journal Article)
The spine is a segmented axial structure made of alternating vertebral bodies (centra) and intervertebral discs (IVDs) assembled around the notochord. Here, we show that, prior to centra formation, the outer epithelial cell layer of the zebrafish notochord, the sheath, segments into alternating domains corresponding to the prospective centra and IVD areas. This process occurs sequentially in an anteroposterior direction via the activation of Notch signaling in alternating segments of the sheath, which transition from cartilaginous to mineralizing domains. Subsequently, osteoblasts are recruited to the mineralized domains of the notochord sheath to form mature centra. Tissue-specific manipulation of Notch signaling in sheath cells produces notochord segmentation defects that are mirrored in the spine. Together, our findings demonstrate that notochord sheath segmentation provides a template for vertebral patterning in the zebrafish spine.
Full Text
Duke Authors
Cited Authors
- Wopat, S; Bagwell, J; Sumigray, KD; Dickson, AL; Huitema, LFA; Poss, KD; Schulte-Merker, S; Bagnat, M
Published Date
- February 20, 2018
Published In
Volume / Issue
- 22 / 8
Start / End Page
- 2026 - 2038
PubMed ID
- 29466731
Pubmed Central ID
- PMC5860813
Electronic International Standard Serial Number (EISSN)
- 2211-1247
Digital Object Identifier (DOI)
- 10.1016/j.celrep.2018.01.084
Language
- eng
Conference Location
- United States