A single, continuous metric to define tiered serum neutralization potency against HIV.

Published online

Journal Article

HIV-1 Envelope (Env) variants are grouped into tiers by their neutralization-sensitivity phenotype. This helped to recognize that tier 1 neutralization responses can be elicited readily, but do not protect against new infections. Tier 3 viruses are the least sensitive to neutralization. Because most circulating viruses are tier 2, vaccines that elicit neutralization responses against them are needed. While tier classification is widely used for viruses, a way to rate serum or antibody neutralization responses in comparable terms is needed. Logistic regression of neutralization outcomes summarizes serum or antibody potency on a continuous, tier-like scale. It also tests significance of the neutralization score, to indicate cases where serum response does not depend on virus tiers. The method can standardize results from different virus panels, and could lead to high-throughput assays, which evaluate a single serum dilution, rather than a dilution series, for more efficient use of limited resources to screen samples from vaccinees.

Full Text

Duke Authors

Cited Authors

  • Hraber, P; Korber, B; Wagh, K; Montefiori, D; Roederer, M

Published Date

  • January 19, 2018

Published In

Volume / Issue

  • 7 /

PubMed ID

  • 29350181

Pubmed Central ID

  • 29350181

Electronic International Standard Serial Number (EISSN)

  • 2050-084X

Digital Object Identifier (DOI)

  • 10.7554/eLife.31805

Language

  • eng

Conference Location

  • England