Development of a neutral embedding resin for optical imaging of fluorescently labeled biological tissue.

Published

Journal Article

Plastic embedding is widely applied in light microscopy analyses. Previous studies have shown that embedding agents and related techniques can greatly affect the quality of biological tissue embedding and fluorescent imaging. Specifically, it is difficult to preserve endogenous fluorescence using currently available acidic commercial embedding resins and related embedding techniques directly. Here, we developed a neutral embedding resin that improved the green fluorescent protein (GFP), yellow fluorescent protein (YFP), and DsRed fluorescent intensity without adjusting the pH value of monomers or reactivating fluorescence in lye. The embedding resin had a high degree of polymerization, and its fluorescence preservation ratios for GFP, YFP, and DsRed were 126.5%, 155.8%, and 218.4%, respectively.

Full Text

Duke Authors

Cited Authors

  • Zhou, H; Gang, Y; Chen, S; Wang, Y; Xiong, Y; Li, L; Yin, F; Liu, Y; Liu, X; Zeng, S

Published Date

  • October 2017

Published In

Volume / Issue

  • 22 / 10

Start / End Page

  • 1 - 7

PubMed ID

  • 29076308

Pubmed Central ID

  • 29076308

Electronic International Standard Serial Number (EISSN)

  • 1560-2281

International Standard Serial Number (ISSN)

  • 1083-3668

Digital Object Identifier (DOI)

  • 10.1117/1.jbo.22.10.106015

Language

  • eng