Wnt5a is necessary for normal kidney development in zebrafish and mice.

Published

Journal Article

Wnt5a is important for the development of various organs and postnatal cellular function. Little is known, however, about the role of Wnt5a in kidney development, although WNT5A mutations were identified in patients with Robinow syndrome, a genetic disease which includes developmental defects in kidneys. Our goal in this study was to determine the role of Wnt5a in kidney development.Whole-mount in situ hybridization was used to establish the expression pattern of Wnt5a during kidney development. Zebrafish with wnt5a knockdown and Wnt5a global knockout mice were used to identify kidney phenotypes.In zebrafish, wnt5a knockdown resulted in glomerular cyst formation and dilated renal tubules. In mice, Wnt5a global knockout resulted in pleiotropic, but severe, kidney phenotypes, including agenesis, fused kidney, hydronephrosis and duplex kidney/ureter.Our data demonstrated the important role of Wnt5a in kidney development. Disrupted Wnt5a resulted in kidney cysts in zebrafish and pleiotropic abnormal kidney development in mice.

Full Text

Duke Authors

Cited Authors

  • Huang, L; Xiao, A; Choi, SY; Kan, Q; Zhou, W; Chacon-Heszele, MF; Ryu, YK; McKenna, S; Zuo, X; Kuruvilla, R; Lipschutz, JH

Published Date

  • January 2014

Published In

Volume / Issue

  • 128 / 1-2

Start / End Page

  • 80 - 88

PubMed ID

  • 25412793

Pubmed Central ID

  • 25412793

Electronic International Standard Serial Number (EISSN)

  • 1660-2129

International Standard Serial Number (ISSN)

  • 1660-8151

Digital Object Identifier (DOI)

  • 10.1159/000368411

Language

  • eng