Skip to main content

Direct Detection of Unamplified Pathogen RNA in Blood Lysate using an Integrated Lab-in-a-Stick Device and Ultrabright SERS Nanorattles.

Publication ,  Journal Article
Ngo, HT; Freedman, E; Odion, RA; Strobbia, P; De Silva Indrasekara, AS; Vohra, P; Taylor, SM; Vo-Dinh, T
Published in: Sci Rep
March 6, 2018

Direct detection of genetic biomarkers in body fluid lysate without target amplification will revolutionize nucleic acid-based diagnostics. However, the low concentration of target sequences makes this goal challenging. We report a method for direct detection of pathogen RNA in blood lysate using a bioassay using surface-enhanced Raman spectroscopy (SERS)-based detection integrated in a "lab-in-a-stick" portable device. Two levels of signal enhancement were employed to achieve the sensitivity required for direct detection. Each target sequence was tagged with an ultrabright SERS-encoded nanorattle with ultrahigh SERS signals, and these tagged target sequences were concentrated into a focused spot for detection using hybridization sandwiches with magnetic microbeads. Furthermore, the washing process was automated by integration into a "lab-in-a-stick" portable device. We could directly detect synthetic target with a limit of detection of 200 fM. More importantly, we detected plasmodium falciparum malaria parasite RNA directly in infected red blood cells lysate. To our knowledge, this is the first report of SERS-based direct detection of pathogen nucleic acid in blood lysate without nucleic acid extraction or target amplification. The results show the potential of our integrated bioassay for field use and point-of-care diagnostics.

Duke Scholars

Altmetric Attention Stats
Dimensions Citation Stats

Published In

Sci Rep

DOI

EISSN

2045-2322

Publication Date

March 6, 2018

Volume

8

Issue

1

Start / End Page

4075

Location

England

Related Subject Headings

  • Spectrum Analysis, Raman
  • Sensitivity and Specificity
  • RNA, Protozoan
  • Point-of-Care Testing
  • Nucleic Acid Hybridization
  • Molecular Diagnostic Techniques
  • Malaria, Falciparum
  • Lab-On-A-Chip Devices
  • Blood Cells
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Ngo, H. T., Freedman, E., Odion, R. A., Strobbia, P., De Silva Indrasekara, A. S., Vohra, P., … Vo-Dinh, T. (2018). Direct Detection of Unamplified Pathogen RNA in Blood Lysate using an Integrated Lab-in-a-Stick Device and Ultrabright SERS Nanorattles. Sci Rep, 8(1), 4075. https://doi.org/10.1038/s41598-018-21615-3
Ngo, Hoan T., Elizabeth Freedman, Ren Abelard Odion, Pietro Strobbia, Agampodi Swarnapali De Silva Indrasekara, Priya Vohra, Steve M. Taylor, and Tuan Vo-Dinh. “Direct Detection of Unamplified Pathogen RNA in Blood Lysate using an Integrated Lab-in-a-Stick Device and Ultrabright SERS Nanorattles.Sci Rep 8, no. 1 (March 6, 2018): 4075. https://doi.org/10.1038/s41598-018-21615-3.
Ngo HT, Freedman E, Odion RA, Strobbia P, De Silva Indrasekara AS, Vohra P, et al. Direct Detection of Unamplified Pathogen RNA in Blood Lysate using an Integrated Lab-in-a-Stick Device and Ultrabright SERS Nanorattles. Sci Rep. 2018 Mar 6;8(1):4075.
Ngo, Hoan T., et al. “Direct Detection of Unamplified Pathogen RNA in Blood Lysate using an Integrated Lab-in-a-Stick Device and Ultrabright SERS Nanorattles.Sci Rep, vol. 8, no. 1, Mar. 2018, p. 4075. Pubmed, doi:10.1038/s41598-018-21615-3.
Ngo HT, Freedman E, Odion RA, Strobbia P, De Silva Indrasekara AS, Vohra P, Taylor SM, Vo-Dinh T. Direct Detection of Unamplified Pathogen RNA in Blood Lysate using an Integrated Lab-in-a-Stick Device and Ultrabright SERS Nanorattles. Sci Rep. 2018 Mar 6;8(1):4075.

Published In

Sci Rep

DOI

EISSN

2045-2322

Publication Date

March 6, 2018

Volume

8

Issue

1

Start / End Page

4075

Location

England

Related Subject Headings

  • Spectrum Analysis, Raman
  • Sensitivity and Specificity
  • RNA, Protozoan
  • Point-of-Care Testing
  • Nucleic Acid Hybridization
  • Molecular Diagnostic Techniques
  • Malaria, Falciparum
  • Lab-On-A-Chip Devices
  • Blood Cells