Consensus recommendations for trabecular meshwork cell isolation, characterization and culture.

Journal Article (Journal Article;Review)

Cultured trabecular meshwork (TM) cells are a valuable model system to study the cellular mechanisms involved in the regulation of conventional outflow resistance and thus intraocular pressure; and their dysfunction resulting in ocular hypertension. In this review, we describe the standard procedures used for the isolation of TM cells from several animal species including humans, and the methods used to validate their identity. Having a set of standard practices for TM cells will increase the scientific rigor when used as a model, and enable other researchers to replicate and build upon previous findings.

Full Text

Duke Authors

Cited Authors

  • Keller, KE; Bhattacharya, SK; Borrás, T; Brunner, TM; Chansangpetch, S; Clark, AF; Dismuke, WM; Du, Y; Elliott, MH; Ethier, CR; Faralli, JA; Freddo, TF; Fuchshofer, R; Giovingo, M; Gong, H; Gonzalez, P; Huang, A; Johnstone, MA; Kaufman, PL; Kelley, MJ; Knepper, PA; Kopczynski, CC; Kuchtey, JG; Kuchtey, RW; Kuehn, MH; Lieberman, RL; Lin, SC; Liton, P; Liu, Y; Lütjen-Drecoll, E; Mao, W; Masis-Solano, M; McDonnell, F; McDowell, CM; Overby, DR; Pattabiraman, PP; Raghunathan, VK; Rao, PV; Rhee, DJ; Chowdhury, UR; Russell, P; Samples, JR; Schwartz, D; Stubbs, EB; Tamm, ER; Tan, JC; Toris, CB; Torrejon, KY; Vranka, JA; Wirtz, MK; Yorio, T; Zhang, J; Zode, GS; Fautsch, MP; Peters, DM; Acott, TS; Stamer, WD

Published Date

  • June 2018

Published In

Volume / Issue

  • 171 /

Start / End Page

  • 164 - 173

PubMed ID

  • 29526795

Pubmed Central ID

  • PMC6042513

Electronic International Standard Serial Number (EISSN)

  • 1096-0007

Digital Object Identifier (DOI)

  • 10.1016/j.exer.2018.03.001


  • eng

Conference Location

  • England