Long-term optical brain imaging in live adult fruit flies.

Published

Journal Article

Time-lapse in vivo microscopy studies of cellular morphology and physiology are crucial toward understanding brain function but have been infeasible in the fruit fly, a key model species. Here we use laser microsurgery to create a chronic fly preparation for repeated imaging of neural architecture and dynamics for up to 50 days. In fly mushroom body neurons, we track axonal boutons for 10 days and record odor-evoked calcium transients over 7 weeks. Further, by using voltage imaging to resolve individual action potentials, we monitor spiking plasticity in dopamine neurons of flies undergoing mechanical stress. After 24 h of stress, PPL1-α'3 but not PPL1-α'2α2 dopamine neurons have elevated spike rates. Overall, our chronic preparation is compatible with a broad range of optical techniques and enables longitudinal studies of many biological questions that could not be addressed before in live flies.

Full Text

Duke Authors

Cited Authors

  • Huang, C; Maxey, JR; Sinha, S; Savall, J; Gong, Y; Schnitzer, MJ

Published Date

  • February 28, 2018

Published In

Volume / Issue

  • 9 / 1

Start / End Page

  • 872 -

PubMed ID

  • 29491443

Pubmed Central ID

  • 29491443

Electronic International Standard Serial Number (EISSN)

  • 2041-1723

International Standard Serial Number (ISSN)

  • 2041-1723

Digital Object Identifier (DOI)

  • 10.1038/s41467-018-02873-1

Language

  • eng