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Quantifying ceramide kinetics in vivo using stable isotope tracers and LC-MS/MS.

Publication ,  Journal Article
Chen, Y; Berejnaia, O; Liu, J; Wang, S-P; Daurio, NA; Yin, W; Mayoral, R; Petrov, A; Kasumov, T; Zhang, G-F; Previs, SF; Kelley, DE; McLaren, DG
Published in: Am J Physiol Endocrinol Metab
September 1, 2018

Numerous studies have implicated dyslipidemia as a key factor in mediating insulin resistance. Ceramides have received special attention since their levels are inversely associated with normal insulin signaling and positively associated with factors that are involved in cardiometabolic disease. Despite the growing literature surrounding ceramide biology, there are limited data regarding the activity of ceramide synthesis and turnover in vivo. Herein, we demonstrate the ability to measure ceramide kinetics by coupling the administration of [2H]water with LC-MS/MS analyses. As a "proof-of-concept" we determined the effect of a diet-induced alteration on ceramide flux; studies also examined the effect of myriocin (a known inhibitor of serine palmitoyltransferase, the first step in sphingosine biosynthesis). Our data suggest that one can estimate ceramide synthesis and draw conclusions regarding the source of fatty acids; we discuss caveats in regards to method development in this area.

Duke Scholars

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Published In

Am J Physiol Endocrinol Metab

DOI

EISSN

1522-1555

Publication Date

September 1, 2018

Volume

315

Issue

3

Start / End Page

E416 / E424

Location

United States

Related Subject Headings

  • Tandem Mass Spectrometry
  • Serine C-Palmitoyltransferase
  • Radioactive Tracers
  • Mice, Inbred C57BL
  • Mice
  • Mass Spectrometry
  • Male
  • Fatty Acids, Monounsaturated
  • Enzyme Inhibitors
  • Endocrinology & Metabolism
 

Citation

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Chen, Y., Berejnaia, O., Liu, J., Wang, S.-P., Daurio, N. A., Yin, W., … McLaren, D. G. (2018). Quantifying ceramide kinetics in vivo using stable isotope tracers and LC-MS/MS. Am J Physiol Endocrinol Metab, 315(3), E416–E424. https://doi.org/10.1152/ajpendo.00457.2017
Chen, Ying, Olga Berejnaia, Jinqi Liu, Sheng-Ping Wang, Natalie A. Daurio, Wu Yin, Rafael Mayoral, et al. “Quantifying ceramide kinetics in vivo using stable isotope tracers and LC-MS/MS.Am J Physiol Endocrinol Metab 315, no. 3 (September 1, 2018): E416–24. https://doi.org/10.1152/ajpendo.00457.2017.
Chen Y, Berejnaia O, Liu J, Wang S-P, Daurio NA, Yin W, et al. Quantifying ceramide kinetics in vivo using stable isotope tracers and LC-MS/MS. Am J Physiol Endocrinol Metab. 2018 Sep 1;315(3):E416–24.
Chen, Ying, et al. “Quantifying ceramide kinetics in vivo using stable isotope tracers and LC-MS/MS.Am J Physiol Endocrinol Metab, vol. 315, no. 3, Sept. 2018, pp. E416–24. Pubmed, doi:10.1152/ajpendo.00457.2017.
Chen Y, Berejnaia O, Liu J, Wang S-P, Daurio NA, Yin W, Mayoral R, Petrov A, Kasumov T, Zhang G-F, Previs SF, Kelley DE, McLaren DG. Quantifying ceramide kinetics in vivo using stable isotope tracers and LC-MS/MS. Am J Physiol Endocrinol Metab. 2018 Sep 1;315(3):E416–E424.

Published In

Am J Physiol Endocrinol Metab

DOI

EISSN

1522-1555

Publication Date

September 1, 2018

Volume

315

Issue

3

Start / End Page

E416 / E424

Location

United States

Related Subject Headings

  • Tandem Mass Spectrometry
  • Serine C-Palmitoyltransferase
  • Radioactive Tracers
  • Mice, Inbred C57BL
  • Mice
  • Mass Spectrometry
  • Male
  • Fatty Acids, Monounsaturated
  • Enzyme Inhibitors
  • Endocrinology & Metabolism