Quantifying ceramide kinetics in vivo using stable isotope tracers and LC-MS/MS.

Published

Journal Article

Numerous studies have implicated dyslipidemia as a key factor in mediating insulin resistance. Ceramides have received special attention since their levels are inversely associated with normal insulin signaling and positively associated with factors that are involved in cardiometabolic disease. Despite the growing literature surrounding ceramide biology, there are limited data regarding the activity of ceramide synthesis and turnover in vivo. Herein, we demonstrate the ability to measure ceramide kinetics by coupling the administration of [2H]water with LC-MS/MS analyses. As a "proof-of-concept" we determined the effect of a diet-induced alteration on ceramide flux; studies also examined the effect of myriocin (a known inhibitor of serine palmitoyltransferase, the first step in sphingosine biosynthesis). Our data suggest that one can estimate ceramide synthesis and draw conclusions regarding the source of fatty acids; we discuss caveats in regards to method development in this area.

Full Text

Duke Authors

Cited Authors

  • Chen, Y; Berejnaia, O; Liu, J; Wang, S-P; Daurio, NA; Yin, W; Mayoral, R; Petrov, A; Kasumov, T; Zhang, G-F; Previs, SF; Kelley, DE; McLaren, DG

Published Date

  • September 1, 2018

Published In

Volume / Issue

  • 315 / 3

Start / End Page

  • E416 - E424

PubMed ID

  • 29509438

Pubmed Central ID

  • 29509438

Electronic International Standard Serial Number (EISSN)

  • 1522-1555

Digital Object Identifier (DOI)

  • 10.1152/ajpendo.00457.2017

Language

  • eng

Conference Location

  • United States