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Ultrarobust Biochips with Metal-Organic Framework Coating for Point-of-Care Diagnosis.

Publication ,  Journal Article
Wang, C; Wang, L; Tadepalli, S; Morrissey, JJ; Kharasch, ED; Naik, RR; Singamaneni, S
Published in: ACS Sens
February 23, 2018

Most biosensors relying on antibodies as recognition elements fail in harsh environment conditions such as elevated temperatures, organic solvents, or proteases because of antibody denaturation, and require strict storage conditions with defined shelf life, thus limiting their applications in point-of-care and resource-limited settings. Here, a metal-organic framework (MOF) encapsulation is utilized to preserve the biofunctionality of antibodies conjugated to nanotransducers. This study investigates several parameters of MOF coating (including growth time, surface morphology, thickness, and precursor concentrations) that determine the preservation efficacy against different protein denaturing conditions in both dry and wet environments. A plasmonic biosensor based on gold nanorods as the nanotransducers is employed as a model biodiagnostic platform. The preservation efficacy attained through MOF encapsulation is compared to two other commonly employed materials (sucrose and silk fibroin). The results show that MOF coating outperforms sucrose and silk fibroin coatings under several harsh conditions including high temperature (80 °C), dimethylformamide, and protease solution, owing to complete encapsulation, stability in wet environment and ease of removal at point-of-use by the MOF. We believe this study will broaden the applicability of this universal approach for preserving different types of on-chip biodiagnostic reagents and biosensors/bioassays, thus extending the benefits of advanced diagnostic technologies in resource-limited settings.

Duke Scholars

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Published In

ACS Sens

DOI

EISSN

2379-3694

Publication Date

February 23, 2018

Volume

3

Issue

2

Start / End Page

342 / 351

Location

United States

Related Subject Headings

  • Point-of-Care Systems
  • Nanotubes
  • Metal-Organic Frameworks
  • Gold
  • Fibroins
  • Biosensing Techniques
  • Antibodies, Immobilized
  • 4009 Electronics, sensors and digital hardware
  • 3401 Analytical chemistry
  • 1007 Nanotechnology
 

Citation

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Wang, C., Wang, L., Tadepalli, S., Morrissey, J. J., Kharasch, E. D., Naik, R. R., & Singamaneni, S. (2018). Ultrarobust Biochips with Metal-Organic Framework Coating for Point-of-Care Diagnosis. ACS Sens, 3(2), 342–351. https://doi.org/10.1021/acssensors.7b00762
Wang, Congzhou, Lu Wang, Sirimuvva Tadepalli, Jeremiah J. Morrissey, Evan D. Kharasch, Rajesh R. Naik, and Srikanth Singamaneni. “Ultrarobust Biochips with Metal-Organic Framework Coating for Point-of-Care Diagnosis.ACS Sens 3, no. 2 (February 23, 2018): 342–51. https://doi.org/10.1021/acssensors.7b00762.
Wang C, Wang L, Tadepalli S, Morrissey JJ, Kharasch ED, Naik RR, et al. Ultrarobust Biochips with Metal-Organic Framework Coating for Point-of-Care Diagnosis. ACS Sens. 2018 Feb 23;3(2):342–51.
Wang, Congzhou, et al. “Ultrarobust Biochips with Metal-Organic Framework Coating for Point-of-Care Diagnosis.ACS Sens, vol. 3, no. 2, Feb. 2018, pp. 342–51. Pubmed, doi:10.1021/acssensors.7b00762.
Wang C, Wang L, Tadepalli S, Morrissey JJ, Kharasch ED, Naik RR, Singamaneni S. Ultrarobust Biochips with Metal-Organic Framework Coating for Point-of-Care Diagnosis. ACS Sens. 2018 Feb 23;3(2):342–351.
Journal cover image

Published In

ACS Sens

DOI

EISSN

2379-3694

Publication Date

February 23, 2018

Volume

3

Issue

2

Start / End Page

342 / 351

Location

United States

Related Subject Headings

  • Point-of-Care Systems
  • Nanotubes
  • Metal-Organic Frameworks
  • Gold
  • Fibroins
  • Biosensing Techniques
  • Antibodies, Immobilized
  • 4009 Electronics, sensors and digital hardware
  • 3401 Analytical chemistry
  • 1007 Nanotechnology