A novel method for source-specific hemoglobin adducts of nitro-polycyclic aromatic hydrocarbons.

Journal Article (Journal Article)

Polycyclic aromatic hydrocarbons (PAH) are ubiquitous air pollutants associated with negative impacts on growth, development and behavior in children. Source-specific biological markers of PAH exposure are needed for targeting interventions to protect children. Nitro-derivatives of PAH can act as markers of exposure to diesel exhaust, gasoline exhaust, or general combustion sources. Using a novel HPLC-APCI-MS/MS detection method, we examined four hemoglobin (Hb) adducts of nitro-PAH metabolites and the Hb adduct of a benzo[a]pyrene (BaP) metabolite in 22 umbilical cord blood samples. The samples were collected from a birth cohort with comprehensive data on prenatal PAH exposure, including prenatal personal air monitoring and DNA adducts in maternal and umbilical cord blood. Using non-parametric analyses, heat maps, and principal component analysis (PCA), we analyzed the relationship between the five Hb adducts and previous PAH measurements, with each measurement representing a different duration of exposure. We found that Hb adducts derived from several diesel-related nitro-PAHs (2-nitrofluorene and 1-nitropyrene) were significantly correlated (r = 0.77, p ≤ 0.0001) and grouped together in PCA. Nitro-PAH derived Hb adducts were largely unrelated to previously collected measures of exposure to a number of PAH parent compounds. These measures need to be validated in a larger sample.

Full Text

Duke Authors

Cited Authors

  • Wheelock, K; Zhang, JJ; McConnell, R; Tang, D; Volk, HE; Wang, Y; Herbstman, JB; Wang, S; Phillips, DH; Camann, D; Gong, J; Perera, F

Published Date

  • March 21, 2018

Published In

PubMed ID

  • 29561551

Pubmed Central ID

  • 29561551

Electronic International Standard Serial Number (EISSN)

  • 2050-7895

International Standard Serial Number (ISSN)

  • 2050-7887

Digital Object Identifier (DOI)

  • 10.1039/c7em00522a


  • eng