Peripherally derived macrophages can engraft the brain independent of irradiation and maintain an identity distinct from microglia.

Journal Article (Journal Article)

Peripherally derived macrophages infiltrate the brain after bone marrow transplantation and during central nervous system (CNS) inflammation. It was initially suggested that these engrafting cells were newly derived microglia and that irradiation was essential for engraftment to occur. However, it remains unclear whether brain-engrafting macrophages (beMφs) acquire a unique phenotype in the brain, whether long-term engraftment may occur without irradiation, and whether brain function is affected by the engrafted cells. In this study, we demonstrate that chronic, partial microglia depletion is sufficient for beMφs to populate the niche and that the presence of beMφs does not alter behavior. Furthermore, beMφs maintain a unique functional and transcriptional identity as compared with microglia. Overall, this study establishes beMφs as a unique CNS cell type and demonstrates that therapeutic engraftment of beMφs may be possible with irradiation-free conditioning regimens.

Full Text

Duke Authors

Cited Authors

  • Cronk, JC; Filiano, AJ; Louveau, A; Marin, I; Marsh, R; Ji, E; Goldman, DH; Smirnov, I; Geraci, N; Acton, S; Overall, CC; Kipnis, J

Published Date

  • June 4, 2018

Published In

Volume / Issue

  • 215 / 6

Start / End Page

  • 1627 - 1647

PubMed ID

  • 29643186

Pubmed Central ID

  • PMC5987928

Electronic International Standard Serial Number (EISSN)

  • 1540-9538

Digital Object Identifier (DOI)

  • 10.1084/jem.20180247


  • eng

Conference Location

  • United States